Reagent-Free Analysis Of HGB In Human Blood Based On FTIR/ATR Spectroscopy

Fourier transform infrared spectroscopy (FTIR) with attenuated total reflection (ATR) technology for its simple, rapid, non-destructive and can be used for real time in situ analysis characteristics has been applied in many fields. Hemoglobin is an important biochemical detection Indicator. The traditional detection methods of hemoglobin usually require chemical reagents, time-consuming and expensive, and destroy life system, it is not a real-time and in situ effective determine method. In this paper, Fourier transform infrared (FTIR) spectrometer and attenuated total reflection (ATR) techniques were applied to establish a newly direct and rapid quantitative determination method of HGB for hemoglobin aqueous solution and human blood hemoglobin (HGB)without any chemical reagent.Respectively using single-point regression, direct PLS, and moving window PLS (MWPLS) method establish the analysis models of hemoglobin aqueous solution and human blood hemoglobin (HGB). The model evaluation indicators main included the root mean squared error of predication (RMSEP) and correlation coefficient of predication (RP), RMSEP was used as the goal of model optimization.The results showed that:MWPLS method had the best prediction effect. we obtain the corresponding starting wavenumber,factor number,RMSEP,Rp and window width of MWPLS model for hemoglobin aqueous solution were 2330 cm-1,24,0.836 mg/mL,0.978 and 420, found the continuous band 2330-1552 cm-1 respectively. At the same time, we also obtain the corresponding starting wavenumber,factor number,RMSEP,Rp and window width of MWPLS model for human blood HGB were 2338 cm-1,5,7.972 g/L,0.915 and 490, found the continuous band 2338-1394 cm-1 respectively. The predicted effect were greatly better than the one of direct PLS on the the the whole spectral collecting region. The model was much simpler and stabler.The selected continuous band of human blood HGB contained the one of HGB aqueous solution. It indicated that the selected continuous bands which also provided reference for designing related special spectrometer.