Empirical Study Of Bone Mesenchymal Stem Cells Differentiation Into Cardiac Cells Induced By IGF-1

objective:Coronary heart disease remains the first cause of morbidity and mortality worldwide. It is known that Cardiomyocytes Can not regenerate after birth. Loss of cardiomyocytes leads to regional contractile dysfunction and necrosis.cardiomyocytes in infarcted ventficular tissues are progressively replaced by fibroblasts and form scar tissues, which leads the contractile function disorder and heart function decrease, then induces congestive heart failure. This experiment will aimed to improve the heart function, amendment cardiomyocytes reconstitution, increase cell number and promote neogenesis blood vessel through transplanting Mesenchymal Stem Cell treated by IGF-1.Methods:The bone marrow stem cells were taken from SD rats respectively. The 3th generation BMSCs were induced for 72 hours differentiation into cardiomyopathy with IGF-1, and immunocytochemistry signed BMSCs by cTnT.30 healthy male SD rats were randomly devided into 3 groups, and heart infarction model were established with ligature blood vessels through open the chest. Group A Was control group,and DMEM of 5mL was transplanted into infarction area though intervention 4 weeks after infarction. In Group B1×108MSCs were transplanted into infarction zone. In Group C1×108 induced MSCs were transplanted. The heart function were measured through Powerlab after transplanting, observed the change of myocardial ischemia by CT, calculated the change of infarction area by the method of TTC.Results: Shape and arrangement of the cells changed after induction with IGF-1. cTnT were expressed three weeks after induction. Expression of cTnT was obvious 48 hours after induction, the structure was dense and striation-like. After transplanted induced cells to infarcted area,as the time gone by, cardiac function of B, C group has improved (P<0.05), this improvement in cardiac function ingroup C is more obvious(vs. the B group, P<0.05). Compared with group A, B, the number of capillaries increased and infarction size dicreased significantly in group C (P<0.05). CT and TTC also showed ischemic area obviously decreased in group C.Conclusions:IGF-1 carl induce MSCs differentiation into cardiomyocytes via and expresse myocardium specificity protein in vitro, transplantion of MSCs induced by BMP-2 call more promot the blood capillary micrangium eregeneration which to amendment heart muscle ischemia and and improve the cardiac function.