The Expression Of Cathepsin S In The Blood Cell Of TDVT Rat

ObjectiveTo detect the vascular tissue of rat femoral vein in traumatic deep vein thrombosis with microarray, Screened out genes which were highly homologous with human being and were differentially expressed in before/peak time of thrombosis formation; focused on cathepsin S of genes which were significantly up-regulated, Re-stablished model of rat TDVT, to verify the expression changes of cathepsin S in the rat blood, study its role played in TDVT.Methods1 Stablished rat TDVD model with vascular clamp and external fixation of hip spica cast.To detect the vascular tissue of rat femoral vein in traumatic deep vein thrombosis with microarray, the obtained data were classified by GO categories and analyzed by Fold Change(FC),Screened out genes which were differentially expressed in before/peak time of thrombosis formation, locked the genes which were highly homologous with human being and were significantly up-regulated in the before/peak-formation of thrombosis formation2 Focused on cathepsin S of genes which was significantly up-regulated, Re-stablished model of rat TDVT, Re-stablished 100 rat models of acute traumatic deep vein thrombosis as follow groups:normal control group (A), thrombosis in the initial phase (B), thrombosis peak (C) and the formation of thrombus group (D).3 Each rat blood samples was gathered at corresponding time, total blood cell RNA of each groups was extracted separately and testing its quality, then expression of rats cathepsin S have been detected in each group by Real-time PCR. 4 Combined physiological role of cathepsin S and its participation role in other diseases, studyed its role played in the TDVT.Results:1 Gene chip data showed that cathepsin S was up-regulated in vein tissue since the injury immediately to the peak of thrombosis. The blast results of homology between human and rat is 92%.2 The result of Real-time PCR showed that cathepsin S was up-regulated respectively at 1.91 and 4.49 fold in the peak of thrombosis group compared with the control group, but no difference between the the control group and non-formation group.3 Cathepsin S is highly homologous between rat and human being. It involved in antigen presentation, apoptosis, matrix degradation and other physiological processes.Conclusion:The expression of Cathepsin S is significantly up-regulated in the vascular tissues and blood cell. Cathepsin S is closely related with the formation of TDVT. cathepsin S may play an important role in TDVT by degradation of extracellular matrixan and promoting endothelial cell apoptosis.