| Research Background:Cervical cancer is not only the second most common cancer among female in the world but also one of the main causes of cancer-related death in the developing countries. After 1990s, as for the reasons such as the changes of social environment and living environment, the rising rate of HPV infection, premature of sexual age and disordered sexual life, the morbidity of cervical cancer has been rising. At present it is still rising, and showing a younger trend, Therefore, it has become a serious threat to lives of female.Domestic and foreign research proves the growth of solid tumor is dependent on angiogenesis, and CD105 as vascular marker can make specific expressions on tumors'blood vessels, moreover, it is independent on tumor cells. Therefore, the MVD value which is marked by CD105 can reflect the vascular proliferation of the tumor stroma, the ability of angiogenesis which induced by tumor cells, then it can reveal the biological action of the tumor to some extent. Now, Domestic and foreign reports mostly focus on some malignant tumor such as small cell lung cancer,breast cancer, rectal cancer. Meanwhile, some clinical data support that the MVD value marked by CD105 has a positive correlation with pathological grades, stage, metastasis, lymph node of some malignant tumors such as: small cell lung cancer,breast cancer, rectal cancer. However, few investigations have been conducted regarding the expression of CD105 in serum and tissue, as well as serum CD105 levels and its correlation with tissue CD105 levels in patients with cervical carcinoma. This experiment investigates the CD105 levels in serum and tissue of cervical cancer through ELISA and Immunohisto chemistry ,and to evaluate the relationship between CD105 in serum and tissue, and to find the mechanism of development and metastasis of cervical cancer. At the same time we hope to find a test method which can indicate the development, metastasis of cervical carcinoma and estimate prognosis. We also expect to find the value of CD105 in serum and tissue in diagnosis and estimating prognosis.Materials and methodsAll of patients were randomly selected for their initial treatment derived from Gynecology department of the second hospital of Jilin University from September 2009 to October 2010, including 11 cases with normal cervix, 8 cases with CINⅡ-Ⅲ(ELISA experiment), 10 cases with CINⅡ-Ⅲ(immunohistochemical method), 36 cases with cervical cancer(without lymph node metastasis) , and 17 cases with cervical cancer(with lymph node metastasis) were diagnosed by pathology.Enzyme-linked immunosorbent assay (ELISA): Blood samples for serum of above 72 cases were obtained by cubits vein puncture after admission immediately, centrifuged at 2800 r/min for 8 minutes, and stored at -20°C until the assay was performed. The expression of CD105 was tested by ELISA.Immunohistochemistry: Most of the 74 cases specimens were obtained immediately after hysterectomy, some specimens were obtained after loop electrosurgical excision procedure(LEEP). All the specimens'segments were stained by immunohistochemical method SP, and then the tumor's microvessel density(MVD)marked by CD105 were counted by the Weidner's method.The statistical software SPSS for Windows (version 17.0; SPSS) was used to perform the statistical analysis. x2 inspection was used in counting data, and t-test was used in measurement data. P value of <0.05 was considered significant.Results:1. In serum: there was no significant difference between normal cervix and CINⅡ-Ⅲ(p>0.05). The serum level of CD105 was lower in patients with normal cervix than in the cervical carcinoma(P<0.05). The serum level of CD105 was lower in patients with CINII-III than in the cervical carcinoma(P<0.05). The serum level of CD105 was lower in patients with cervical cancer(without lymph node metastasis ) than in the cervical cancer(with lymph node metastasis ) ,The serum level of CD105 was higher in patients with tumor greater than four centimeters in diameter than in the tumor smaller than four centimeters in diameter, there are significant differences between them(P<0.05). The serum level of CD105 was higher in cervical carcinoma with vascular space invasion than that without vascular space invasion(P<0.05).2.In tissue: The MVD value marked by CD105 value ascend ssuccessively amang normal cervical tissue, CIN and cervical cancer,there are significant difference between them(P<0.05). The MVD value marked by CD105 was higher in cervical carcinoma with lymph node metastasis than that without lymph node metastasis(P<0.05). The MVD value marked by CD105 was higher in patients with tumor greater than four centimeters in diameter than in the tumor smaller than four centimeters in diameter, there are significant differences between them(P<0.05). The MVD value marked by CD105 was higher in patients with tumor deeper than one half in depth than in the tumor shallower than one half in depth, there are significant differences between them(P<0.05).The MVD value marked by CD105 was higher in cervical carcinoma with vascular space invasion than that without vascular space invasion(P<0.05).3. The level of serum CD105 changed as same as the MVD value marked by CD105 in tissue,relative index is 0.683.Conclusion:1. The expression of CD105 was high in cervical carcinoma. This indicates that high expression of CD105 may correlate with the malignancy of cervical carcinoma. Detection the level of CD105 may be one of the indicators which can diagnose the early cervical cancer.2. The expression of CD105 correlates with tumour diameter, vascular space invasion and lymph node metastasis. Detection of the level of CD105 can provide external basis on predicting lymphangitic metastasis and estimating prognosis..3. In an statistical analysis, The level of serum CD105 changed as same as the MVD value marked by CD105 in tissue. There was a positive correlation between them. It was possible that the detection of serum CD105 would reflect the expression of CD105 in tissue, and it may be a simple and inexpensive method used in clinical which can detect CD105 recurrence of cervical cancer.The result of above experiment indicates the expression of CD105 may correlate with malignancy development. Clinicians can evaluate the Tumor load conditions and metastasis through detecting the level of CD105 in serum and tissue to provide an external basis for diagnosis and personal therapy. Detection of serum CD105 may take place of tissue CD105 detection and it will be a secure, noninvasive and repeatable detective method. Blocking the expression of CD105 or linking chemotherapy drugs, biologic toxin, radionuclide etc can achieve the purpose of tumor therapy which providing a potential molecular target for therapy of cervical carcinoma.
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