The Effect Of Lactoferrin On The Secretion Of IL-2 And TNF-α By Polymorphonuclear Leukocytes

Periodontal disease is a multifactorial disease, plaque invasion of microbes and their products is the initiating factor of periodontal disease. It is caused by multiple microbes, even the whole ecosystems have influence on it. The periodontal tissue's destruction of Periodontitis patients, is not only caused by the activity of microbial plaque, but also with the excessive inflammatory response of polymorphonuclear leukocytes (PMN). In the inflammatory response, PMN in the junctional epithelium and gingival sulcus, as a the main defense cells, play an important role in the process of defense against external factors.PMN works on the bacteria by releasing the lysosomal enzymes and superoxide ions. Meanwhile, they also release various of inflammatory factors to keep themselves over activated through feedback mechanisms, so to expand their effective period. As a result, they release more inflammatory factors, and finally cause the necrosis of PMN, releasing large amounts of proteolytic enzymes and substances, leading to inflammation and local tissue persistence of excessive damage.In this activity, PMN play an important role,IL-2 and TNF-a are two important factors.Porphyromonas gingivalis is widely recognized as the main bacteria leading to periodontal disease,. It is the main advantages of bacteria in lesions or active portion of periodontal disease, especially chronic periodontitis. It produces multiple virulence factors, which damaging the periodontal tissue, such as gingipains, collagenase, peptidases, endotoxin, acid and alkaline phosphatase.In this study, on the basis of characters of anti-bacterial and immune regulation, we try to observe the influence of lactoferrin (LF) on the secretion of IL-2 and TNF-a of PMN stimulated by the bacterial suspension, and try to discuss whether LF have the inhibiting influence on the secretion of IL-2 and TNF-a of PMN, and try to indicate the role of LF in the periodontal tissue. colonies in 48h, centrifuge, extract ultrasonic bacterial. isolate peripheral blood PMN by Percoll discontinuous gradient centrifugation,Join P.g PMN ultrasound filtrate solution of LF in the experimental group, join PMN and PBS Aa ultrasound filtrate solution in the control groupl. Add PMN and PBS solution in the control group 2, detecte secretion levels of IL-2 and TNF-a after training 20min,40min, and 60min by Radioimmunoassay.Results:Under the influence of the LF,the level of IL-2 and TNF-a secreted by the PMN in the experimental group was significantly lower than the control-groupl,which was not added LF, (p<0.05), with statistical Significance.Conclusions:1.LF inhibits the secretion of IL-2 and TNF-a of PMN stimulated by the bacterial suspension.2. In concentrations of 0.1 mg/ml,1 mg/ml,10 mg/ml,the inhibiting effect of LF to IL-2 and TNF-a enhances along with the increasing of the concentration.