| Background:the development of glomerulosclerosis is the DN to renal failure, the underlying pathology. Studies have shown, DN exists in glomerular cells in glomerular sclerosis increased apoptosis, then how to reduce the glomerular cells to achieve anti Jiner is the role of glomerular sclerosis subjects to be studied. Minocycline is a second generation semi-synthetic broad-spectrum antibiotic tetracycline. In addition to its good antibacterial activity, but also has strong anti-apoptosis. The anti-apoptotic role has been in the nervous system diseases, renal ischemia reperfusion injury, and other areas to be confirmed, and in diabetic nephropathy and clinical application of basic research has not been relevant reports.Objective:This study used DN model was minocycline DN apoptosis of rat kidney to determine bcl-2, bax, and caspase-3 expression, a preliminary study of minocycline against apoptosis mechanism.Methods:This project uses animal experiments, the 50 Wistar rats were divided into five groups, namely (1) DM groups:model group (2) M2 short-term treatment group:DM rats as model after 8 weeks oral treatment given minocucline 20mg/Kg, for 8 weeks. (3) M4 continuous treatment group:DM into a mold to give minocucline20mg/Kg given after oral treatment, treatment for 16 weeks. (4) NM groups:normal rats were given oral treatment minocucline 20mg/Kg, treatment for 16 weeks. (5) control group. Biochemical urine testing, rats were killed after four months, taking the left half of kidney is made of wax block, the other half frozen. Pas staining and HE staining using the method of pathological changes observed by light microscopy, and detected by RT-PCR in renal tissue apoptosis gene bcl-2, bax, and caspase-3 mRNA, detected by TUNEL cells in renal tissue DN Apoptosis.Results:The control group in good spirits, action freely, responsive, shiny hair. Model group, apathetic, slow to react, more food, more drinking, urination, significant weight loss, dull hair messy, lying curled hair shaft. Mental state administration group, the reaction, hair worse than the control group, but better than the model group.Model rats increased urine volume, urinary protein was significantly increased, impaired renal function. Compared with model group, minocycline group decreased urine output, renal function, mild urinary protein decreased.Light microscope:the control group and the NM groups:light microscopy glomerular volume, shape and structure were normal. Model group:light microscopy glomerular volume increases, the size of the uneven part of the glomerular capillary loop collapse, widening of mesangial areas, the ball inside the area as part of PAS staining of block, the basement membrane is not Homogeneous thickening. Local vacuolar degeneration of renal tubular epithelial cells, there hyalinization. M2-M4 groups:mild pathological changes.TUNEL labeled apoptotic cells in the tips:the control group had trace amounts of apoptotic cells. NM group of apoptotic cells than the control group increased slightly but not significantly different (P> 0.05), model group, apoptotic cells in renal tissue were markedly increased (P<0.05), M2, M4 renal Apoptotic cells in tissues than the control group (P<0.05), M2, M4 apoptotic cells in renal tissues was significantly less than model group and model group were significant differences (P<0.05);RT-PCR method:bcl-2 in the control group, weak expression, NM group than in the control group increased slightly (P> 0.05), renal tissue of model group than the control group, bcl-2 expression decreased (P<0.05), M2, M4 expression of BCL-2 group than the control group but not statistically significant (P>0.05), M2, M4 group of BCL-2 expression was significantly more than the model group and model group were significant differences (P <0.05); Bax mRNA expression in the control group had a small amount of the model group significantly increased bax expression (P<0.05) M2, M4 group bax expression decreased significantly compared with model group, significant difference (P<0.05); caspase-3 mRNA expression Model group was significantly more than the control group and NM group, treatment group M2, M4 little difference between the two, caspase-3 mRNA treated group was significantly less than model group (P<0.05).Discussion:Diabetic nephropathy (DN) later showed glomerular sclerosis, a number of studies have shown that excessive apoptosis of glomerular sclerosis in the development process plays a key role. B-cell lymphoma/ leukemia 2 gene (bcl-2) regulation of apoptosis, bax is a set of genes, bcl-2 is the common pathway of various apoptosis regulatory genes, the function is suppression of apoptosis. bax bcl-2 is antagonized pro-apoptotic factor. Cysteine protease-3 (caspase-3) is the apoptosis promoting gene, is the most important apoptosis protease, studies have shown that these three genes involved in DN cells. Minocycline (2nd generation semi-synthetic tetracycline drug) In addition to antibacterial effects, but also has strong anti-apoptosis, and its mechanism in nervous system diseases, ischemic disease, diabetic retinopathy was confirmed by animal experiments.This experiment confirmed the existence of diabetic nephropathy excessive apoptosis, and minocycline in the DN model, and thus also has anti-apoptotic effect of anti-glomerular sclerosis, confirmed that minocycline can be adjusted by BCL-2, Bax, caspase-3 expression to play a role in anti-apoptosis. DN provided for the clinical treatment of new ideas. Conclusion:1, DN minocycline can reduce the pathological changes in rats;2, minocycline can play in diabetic nephropathy anti-apoptotic role3, minocycline can inhibit bax, caspase-3 expression, and promote the expression of bcl-2, and play its role in anti-apoptosis4, minocycline has a therapeutic effect on the DN, not only for the treatment of DN provides a new way, but also for the clinical application of minocycline to provide a theoretical basis for anti-apoptosis.
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