| Objective : By observing and Statistical analyzing the morphology, pathology, fibroblasts and the micro vessel density of the rabbits'ears scar models which have fed different doses of Citric Tamoxifen tables to research the effects of the Estrogen antagonist on rabbits'ears scar models, so as to provide the thinking of new methods to prevent and treat the skin scars in Clinical Medicine. Methods: 1. Experimental Design: Randomized controlled animal study;2. Object of study:9 Japanese rabbits,Female,3 to 6 months old and the weight is between 1.5 and 2 kg;3. Study methods: Production the rabbits'ears scar models after intramuscular anesthesia with 2mg/Kg Ketamine. Each rabbit ear have been made 4 models, the total is 72. Then, divide these 9 rabbits to three groups by random method. The group A is blank group, group B is fed with 10mg/kg/d of Citric Tamoxifen tables, and the group C is fed with 5mg/kg/d of Citric Tamoxifen tables. Use the N.S to protect the ear cuts until all the cuts are healing. We must comply with the aseptic operation during the surgery and treatment process. Observe the formation of the rabbits'ears scar. On the 29 days after the surgery, we execute all the rabbits to get the rabbits'ears scar organization to do HE coloring and Immunohistochemical staining of CD34, then, observed the pathological changes and count the number of fibroblasts and the micro vessel density of these scar organization. At the same time , we make the uterus organization pathological examination. All the results and pictures are analyzed by spss13.0 software. Results: 1. Morphological results of general observation : The blank group's color is more darker, texture is hard, we can touch the scleroma under the skin, the surface is not smooth, the vicinity scar is uplifting very much. The group B and group C's color is lighter than group A, close to skin color, the texture is softer and the scleroma is smaller. 2. histological results of general observation: HE staining observed in blank group scar tissue sections marked capillary proliferation, large fibroblast nuclears, dense hyperplasia, in full-thickness dermal collagen disordered;Group B's scar tissue are thinner, surface is flat, level is in order, fibroblasts significantly reduced;The thickness and hardness of the scar tissue of the group C is between the group A and the group B, under the light microscope, we find the hyperplasia of fibroblasts, a part of nucleus become large, the order of the fibroblasts is not as the group B, and the hyperplasia of micro vessel is also between group A and group B, the range is more tightly. We did the uterine pathological section and find that the group B's uterine lining and glands have slight hyperplasia; The change of the uterus organization in group C is not obvious, we do not find the uterus membrane and glands have obvious hyperplasia. 3. fibroblasts number: The fibroblasts number of group A and group B is less compared to the blank group, the difference during three groups has Statistical significance; when the group A is compared with the group B, the difference between these two groups also has Statistical significance, so we can consider that the 10mg/kg/d of Citric Tamoxifen is more effect than 5mg/kg/d of Citric Tamoxifen on inhibiting the growth of fibroblasts. 4. micro vessel density: Immunohistochemistry staining of CD34 find that The micro vessel density of group A and group B is less compared to the blank group, the difference during three groups has Statistical significance; when the group A is compared to the group B ,the difference between these two groups also has Statistical significance, so we can consider that the 10mg/kg/d of Citric Tamoxifen is more effect than 5mg/kg/d of Citric Tamoxifen on inhibiting the growth of micro vessel.Conclusions: Estrogen antagonist (Citric Tamoxifen tables) has the obvious effect on inhibiting the growth of skin scars. the 10mg/kg/d of Citric Tamoxifen is more effect than 5mg/kg/d of Citric Tamoxifen on inhibiting the growth of fibroblasts and micro vessel. The mechanism is maybe related to the Estrogen antagonist can inhibit the growth of fibroblasts and micro vessel. |
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