| Objective:Analysis and comparison LGT model rat serum and tumor tissue LGT(LGT-LostGoodwill Target)protein expression of fingerprint characteristics.Methods:Take 4-5 weeks female mice 1 only,a mouse model was developed by inoculatingS180 cells directly into KM mice. Tumor cell S180 with ascites tumor form in kunming (KM)mice nestin, the first eight days, after nestin under aseptic conditions and to extract ascites, adjustthe diluted saline cell density 2 * 107 / ml, make tumor levitation liquid, on the ice bath. Setaside. Will preparation good tumor cell suspension liquid per only 0.2 ml in kunming mice (10)only right armpit subcutaneous inoculation made moulds. All the mice were divided into 2groups. When the tumor long to 0.5 cm big hours ,group A was injected cisplatin 5.2 mg/kg/d for4 days by intraperitoneal injection. Group B was control groups for 4 days by with group A ofintraperitoneal injection with days such as measuring saline * 4 days . The mice were killed atthe fourth day after chemotherapy end. Then we collected the eyeball blood samples, and SELDIwas applicated to test it. Made of module mice anatomy, were taken tumor tissue, peritumoralorganization and normal tissue 1g each, according to 0.5 g organization to join 1ml physiologicalsaline, use electric homogenate device homogenate, 3000r/min centrifugal separation 5minZuZhiYe, extraction supernatant fluid lines SELDI inspection. Respective proteomic fingerprintwas analyzed by Biomarker Wizard software to discover the different proteomic fingerprints.Results①Group A serum LGT proteome fingerprint is positive, group B LGT serum proteinfingerprint negative.②Group A serum protein with cancer organization fingerprint: In the detection of proteinfingerprint: there are 9 protein difference was statistically significant (P < 0. 05). Differencesprotein fingerprint m/z respectively:1993,2009,1028,2217,2502,2545,8696,9940,10145。③Group A serum protein with peritumoral organization fingerprint:In the detection of proteinfingerprint: there are 7 protein difference was statistically significant (P < 0. 05). Differencesprotein fingerprint m/z respectively:1077,1211,1994,2009,2030,2081,11817。④Group A serum protein with normal tissue fingerprint:In the detection of protein fingerprint:there are 5 protein difference was statistically significant (P < 0. 05). Differences proteinfingerprint m/z respectively:1004,1994,2010,2030,2217。⑤Group A cancer organization with normal tissue fingerprint:In the detection of proteinfingerprint: there are 5 protein difference was statistically significant (P < 0. 05). Differencesprotein fingerprint m/z respectively:2545,3120,3344,4958,5915,7489。⑥Group A cancer organization with peritumoral organization fingerprint:In the detection ofprotein fingerprint: there are 6 protein difference was statistically significant (P < 0. 05).Differences protein fingerprint m/z respectively:1224,1994,2030,3120,11817,15167。⑦Group A peritumoral organization with normal tissue fingerprint:In the detection of proteinfingerprint: there are 6 protein difference was statistically significant (P < 0. 05). Differencesprotein fingerprint m/z respectively:2030,4958,5915,7492,10144,11819。⑧Located in m/z: 11,000 + H ~ 12,000 + H in exp1 distinguish as potential biomarkersscreening out, From serum group, cancerous tissue group, cancer tissue adjacent group to normal tissue group, each in turn the expression rate rise: Its abundance serum group (3.6), canceroustissue group (17.9), cancer tissue adjacent group (10.2), and normal tissue group (63.4). ZuZhiYein normal LGT proteins relative content is the highest.ConclusionConclusion: LGT-Lost Goodwill Target Exists in cancerous tissue, cancer tissue adjacent,normal tissues, Especially in normal tissue for high expression (63.4). This conclusion is causedby tumor progression and cause death occurred factors likely not tumor itself, in yoursurroundings. Each place of expression protein fingerprints are not uniform, its and LGTrelationship and significance to research further.
|
PDF Full Text Request