The Expression And Amplification Of EGFR,HER2 And TOPOⅡ In Esophageal Canceration Course

ObjectiObjective1. To investigate the expression of EGFR,HER2 and TOPOⅡin esophageal cancerationcourse,analyze the correlation between the expression and clinical pathological parameters, andanalyze the correlation of the three gene.2. To investigate the gene status of EGFR,HER2 and TOPOⅡin esophageal squamous cellcarcinoma(ESCC),analyze the correlation between the gene status and clinical pathologicalparameters, analyze and the correlation of the three gene.3. Through comparision of EGFR,HER2 and TOPOⅡprotein/gene status detected byimmunohistochemistry(IHC), chromogenic in-situ hybridization(CISH), to explore the clinicalsignificance of IHC and CISH on detecting EGFR,HER2 and TOPOⅡgene status.Methods1.Collecting 107 cases of esophageal carcinoma, including normal esophageal epithelium,esophageal intraepithelial neoplasia and esophageal squamous cell carcinoma(ESCC). Tissuemicroarray was made by Tissue microarray technology.2.Envision immunohistochemisty method was performed to analyze the expression ofEGFR,HER2 and TOPOⅡin esophageal canceration course.3.CISH was performed to analyze the gene Status of EGFR,HER2 and TOPOⅡin ESCC.4.FISH was performed to analyze the HER2 gene Status in ESCC.Results1. The expression of EGFR, HER2 and TOPOⅡin esophageal canceration course.The positive expression rates of EGFR and TOPOⅡdisplay a improving trend fromnormal esophageal epithelium,intraepithelial neoplasia to carcinoma(P<0.005), in the abovethree groups,The positive expression rates of EGFR were 8.41%,15.89%,50.47%, andTOPOⅡwere 3.74%,11.21%,43.93%.Furthermore,the expression showed gradually incresingwith histological grades advancing(P<0.01). there was no correlation between EGFR orTOPOⅡand gender,age,lymph node or distant metastasis(P>0.05).There was a positivecorrelation between EGFR and TOPOⅡ(r蛋白1=0.410,P<0.05). Among the three groups,thepositive expression rates of HER2 were 0%, 1.87%, 1.87%. There were no significance of HER2protein expression among the three groups(P>0.05), no correlation was obtained between HER2and gender,age,the depth of invasion, histological grades or lymph node metastasis(P>0.05), norelationship between HER2 and EGFR or TOPOⅡ. 2. The gene status of EGFR,HER2 and TOPOⅡdetected by CISH in ESCC.In ESCC,EGFR gene amplifieation was found in 35(32.71%)cases,TOPOⅡwas 31(28.97%)cases.EGFR,TOPOⅡpositive was significantly correlated with histologicalgrades,and no correlation between EGFR or TOPOⅡand gender,age,lymph node or distantmetastasis(P>0.05).There was a positive correlation between EGFR and TOPOⅡ( r基因= 0.530,P<0.05). HER2 gene amplifieation was found in 2(1.87%)cases, and no correlation wasobtained between HER2 and gender,age,the depth of invasion, histological grades or lymph nodemetastasis(P>0.05), no relationship between HER2 and EGFR or TOPOⅡ(r基因2= 0.23 1,P>0.05;r基因3=0.169,P>0.05).3. Comparision of EGFR,HER2 and TOPOⅡprotein/gene status detected by IHC, CISH.In ESCC, the general coincident rate of EGFR or TOPOⅡdetected by IHC,CISH was64.81%(35/54)and 65.95%(31/47). Amongst the cases of EGFR or TOPOⅡproteinoverexpression (+++) group,the concordant ratios of IHC and CISH detecting EGFR or TOPOⅡstatus were 80% and 83.33%; amongst the cases of EGFR or TOPOⅡprotein overexpression(++) group, the concordant ratios were 58.97% and 60%; amongst the cases of EGFR orTOPOⅡprotein expression (+/-) group, the concordant ratios were both 100%. The concordantratios of HER2 detected by IHC, CISH was 100%．4. The gene status of HER2 detected by FISH.Though FISH detected HER2 gene Status in 107 cases of ESCC, gene amplifieation wasfound in 1 case,others were no gene amplifieation,incluing 1 case detected Chromosome 17polysomy.Conclusion1. EGFR and TOPOⅡare closely related to the occurrence and development ofesophageal squamous cell carcinoma.their expressions all make a qualitative change in theesophageal high-grade intraepithelial neoplasia. The role of HER2 in the development ofesophageal squamous cell carcinoma is still not definite in ShanXi provice.2. IHC is a preliminary screening method by detecting EGFR and TOPOⅡprotein.Because protein expression and gene amplification test results exsist some differences, we needto select CISH further for IHC(++~+++).3．Compared with the CISH, FISH play an improtant role in the detection of HER2 geneon Chromosome 17 polysomy.