The Effect Of Exogenous IGFBPrP1 On Liver And Kidney Of Mice

Objective:To investigate the effect of exogenous recombinant mouse insulin-like growth factor binding protein related protein 1 (rmIGFBPrP1) on mouse liver and kidney tissue, explore the mechanism of rmIGFBPrP1 on liver and kidney fibrosis.Methods:A total of 26 male wild-type C57BL/6 mice were randomly divided into normal control group, sham-operated group and model group. We used rmIGFBPrP1 which was released by capsule osmotic pump to make the model. HE staining and Sirius red staining were observed to investigate the pathological changes of liver and kidney of mice,deposition of collagen fibers. Detect the expression and distribution of IGFBPrP1, Smad3, p-Smad2/3,â…¢collagen (Collagenâ…¢) of liver and kidney tissues by immunohistochemistry. Detect hepatocyte apoptosis by DUTP nick end labeling (TUNEL).Results:Comparing with the control group and sham-operated group, the content of collagen fiber increased, expression of IGFBPrPl, Smad3, p-Smad2/3, Collagenâ…¢increased (P<0.01) in the liver of model group. Only the expression of IGFBPrP1 in the kidney of model group increased than the control group and sham-operated group(P<0.01), there is no difference on collagen fibers content and Other indicators expression(P>0.05). TUNEL showed an increase in the number of apoptotic liver cells in model group than the normal group and sham-operated group, the difference was statistically significant (P<0.01).Conclusions:1. Exogenous IGFBPrP1 can promote hepatocyte apoptosis and liver fibrosis, but have little effect on the mice kidney.2. IGFBPrP1 may has the selectivity on inducing organs and organization fibrosis of mouse.