| Objective To study the influence of HA-VEGF carrier to the survival rate of the skin and fatgrafting and graft vascular regeneration, investigate the delay-release effect of HA to VEGF andVEGF amplification of biological effects..Methods Physiological saline ,VEGF and HA-VEGF were respectively acted as blank,control and experimental group.72 rats were divided into 24 groups(3 rats as a group, each ratwas used 2 different reagents).The dorsal of rats was divided into four in average, doing the skingrafting near the caudal (to get the whole skin graft about 2cm in diameter in the left and rightsides and exchange the position),the group of drugs were respectively injected into rat skin graftsand implant bed. Doing the fat grafting as follow:getting fat about 0.2g in the inguinal of rats andtransplant near the cephalic in the rat dorsal, the group of drugs were respectively injected intofat. Counting the survival rate of graft at different times; detecting the regeneration of capillariesby HE staining and testing the the expression of CD34 by immunohistochemical staining.Results1. The survival area, the change of weight ,the survival rate in the grafting skin and the graftingfat in the HA-VEGF group were all significantly higher than the other controls(P<0.05);andit was significantly difference between VEGF group and blank.2. The number of capillary inthe grafting skin and the grafting fat were all significantly higherthan the other controls(P<0.05);and it was significantly difference between VEGF group andblank.3. The expression of CD34 inthe grafting skin and the grafting fat were all significantly higherthan the other controls(P<0.05);and it was significantly difference between VEGF group andblank.Conclusion HA had a delayed release effect to VEGF. It can increase VEGF retained time inthe local tissue and slow down the degradation of VEGF, so that VEGF can induce the formationof new blood capillaries to increase blood flow and promote the survival of skin grafts.
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