Kistrin Interfere In FAK And ERK Signal Factor In The Early Stage On Rabbit's PCO Model

Objective: To evaluate the gene expressions of FAK and ERK in the lens epithelial cells on the rabbits posterior capsular opacification (PCO) models with kistrin interference and investigate the possible mechanisms of this procedure. Method: Extracapsular lens extraction were performed on right eyes of eight New Zealand white rabbits to establish the of PCO models. Eight right eyes were randomly divided into experimental group and control group, each group including four eyes. At the end of the operations, 0.2ml of kistrin (80μg·L ^-1) were injected into the capsular bags of the experimental group while 0.2ml of Ringer's irrigation were injected into the control group. Four left eyes were chosen randomly and worked as the blank group. At 1^st, 2^nd, 3^rd, 7^th and 14^th day after surgery, the conjunctiva, cornea, anterior chamber and the posterior capsule were examined by slit-lamp microscopy and the fundus conditions were observed by the funduscope. The residual lens capsules were taken out at the 14th day. The RNA of the cells of the posterior capsules from the three groups was extracted and the mRNA expressions of FAK and ERK were evaluated by real-time PCR. All quantitative data were represented by mean (x|⁾±SD(standard deviation), Mean in groups was compared with single factor variance analysis;The rank sum test was used for ranked data. Results: (1) The anterior segment was observed by slit-lamp after ECLE. We found different degree conjunctiva congestion and edema, corneal edema and turbid aqueous humor in both control group and experimental group 3^rd day after surgery, showing no difference. Fundus condition can't be examined in both groups. At 7^th day after surgery, corneas recovered to clearness but aqueous flare and micro fibrinous exudates can be observed in some eyes. We found that all the eyes have transparent corneas and clear aqueous humor, and the fibrinous exudates were absorbed at 14^th day. PCO condition: At 7^th day after surgery, both groups have no PCO, belong to degree zero; At 14^th day, two eyes of degree zero and one eye of degree one in the control group, three eyes of degree zero and one eye of degree one in the experimental group. There was no difference between the two groups by the rank sum test (p>0.05). (2) The normal rabbit residual lens capsules after ECLE expressed mRNA of FAK and ERK, the abundance were 1.476±0.802,0.576±0.035 respectively. At 14^th day after surgery, the abundance of FAK mRNA and ERK mRNA were 6.496±1.501,1.158±0.573 in the control group, while in the experimental group6.06±1.512,1.262±0.329 in the experimental group. The expressions of FAK mRNA and ERK mRNA increased significantly in the control group and the experimental group compared with the blank group (p<0.05). There was no difference between the experimental and the control group (p>0.05).Conclusions: The LECs express more mRNA of FAK and ERK after extracapsular lens extraction. FAK-ERK signal pathway participated in the development of PCO. Administration of Kistrin didn't change FAK and ERK gene expression of LECs at mRNA level.