Radix Astragali For AGEs Glomerular Mesangial Cells Damage In Vitro Destruction Of Intervention

Objective: to study the affect of AGEs on proliferation, apoptosis and memebrace surface negative charge of GMC of cows, then to analyze the protection of Radix Astragali on GMC in vitro.Methods: 1. the affect of AGEs on proliferation of GMC of cows which were cultured by AGEs in vitro were analyzed with MTT assay. 2. The affect of Radix Astragali on the injury and the changes of surface charge of membrane caused by AGEs were observed by measuring the quality of the membrane of GMC blinding to Alcain Blue. 3. The apoptosis of GMC caused by AGEs was detected by AO/EB Double Staining, and the inhibition of apoptosis by Radix Astragali was evaluated.Results:1. The effect of AGEs on GMC: Proliferation was measured by potical density, OD, the higher the OD was the more the proliferation was. The proliferation of GMC in AGEs group was significantly increased than that in control group at both 24h and 48h (p<0.05); the proliferation of GMC in 0.25 subgroup and 0.5 subgroup was signififcantly increased than that in 0.125 subgroup separately in 24h group (P<0.05); the proliferation of GMC among 0.25 subgroup, 0.5 subgroup and 0.125 subgroup was obviously increased and statistically different from that of each other in 48h group (P<0.05). Then the subgroup, GMC cultured by AGEs on the concentration of 0.25mg/ml for 48h, was suitable for our expenrient.2. The effect of AGEs on proliferation of GMC: Proliferation was measured by potical density. Compared with dulbecco's modified eagle medium,DMEM, the OD of GMC in Radix Astragali group was low and that in AGEs group was high (P<0.05).Compared with ACEs group, the OD of GMC in Radix Astragali group was decreased, and it meaned the proliferation was decreased in Radix Astragali group than AGEs group (P<0.05). The high concentration (0.2 mg/ml) was the most efficient compared with other concentrations (P<0.05). AGEs strongly prompt the prolifecation of GMC of cow, whereas Radix Astragali in all consentrations could inhibit the proliferation, and the OD of GMC in the high concentration (0.2 mg/ml) was lowest, which meaned the inhibihation was the most strong.3. The effect of AGEs on surface negative charge of GMC of cow: the IOD of GMC was lower in AGEs group than that in control group (P<0.05), and it meaned that the memabrane surface charge decreased significantly. Compared with AGEs group, the IODs of different concentrations Radix Astragali subgroups were higher (P<0.05), it meaned the increase of memabrane surface charge. Furthermore, the IOD of high concentration of Radix Astragali subgroup was statistically higher than subgroups of low concentrations (P<0.05). The results showed AGEs decrease the quality of normal GMC blinding to Alcain Blue and Radix Astragali could inhibit it in a dose– dependent manner.4. The effect of Radix Astragali on inhibition of the apoptosis of GMC reduced by AGEs: the apoptosis of GMC in Radix Astragali group of different consentrations was statistically decreased than AGEs group (P<0.05), Moreover, were statistically different among Radix Astragali subgroups of different concentrations. The apoptosis of GMC decreased with the concentration of Radix Astragali increased in a dose - dependent manner.Conclusions: AGEs strongly prompt the prolifecation of GMC of cow; whereas Radix Astragali could inhibit the proliferation induced by AGEs, resist the decrease of surface negative charge of GMC caused by AGEs, and against the apoptosis of GMC reduced by AGEs. In a word, Radix Astragali can protect the GMC in many ways.