Expression Of Correlated Inflammation Mediators On Different Time Phases In Rats With Limbs Ischemia-Reperfusion

Objective In order to supply experimental evidence and rationale to prevent and intervent limbs ischemia-reperfusion in clinic, we investigate the expression and changes of correlated inflammation factors as the time extends in the stage of ischemia reperfusion in rats with right lower limb ischemia-reperfusion and study not only the pathogenesis of limb ischemia-reperfusion but also explore its significance on remote organs injury.Methods Adopting model of rats with right lower limb ischemia-reperfusion,50 Sprague-Dawley rats were randomly divided into the sham operated group and the ischemia-reperfusion group (IR group),each group was divided into 5 groups including 3hours,6hours,9hours,12hours and 15 hours after reperfusion,and each small group contains 5 rats.Expression of IL-1βand IL-6 in blood serum were determined by ELISA,while TNF-αMPO,NF-κB in lung tissue by immunohistochemistry.Result Compared with the sham group, Expression of IL-1βand IL-6 in blood serum and TNF-α,MPO,NF-κB in lung tissue increased on different time phases in the IR group (P<0.05),each factors in the IR group got a higher expression as time prolonged,with IL-1β,IL-6,TNF-a and MPO reached the peak value in 12hours-group while NF-κB in 9hours-group (P<0.05).Expression of each each inflammation factor in the sham group had no significant change (P>0.05)Conclusion By studying the expression and changes of correlated inflammation factors as the time extends in rats with limbs ischemia-reperfusion,dropout and liberation of multi-inflammation factors in represent of IL-1β,IL-6,TNF-αMPO and NF-κB may participate in the mechanism of remote organs injury,supplying experimental evidence and rationale to intervent and prevent limbs ischemia-reperfusion in clinic.