The Protective Effect Of The Reperfusion After Focal Cerebral Ischemia Of The Rat By Naoxintong

The inflammation following cerebral ischemia-reperfusion plays an important role in the brain injury. People pays more and more attention to it these years. Microglia, one of the neuroglia cells, is main immunological cell in the brain, can release inflammatory factors such as turner necrosis factor-α(TNF-α), interleukin-1β(IL-1β) when activated. These factors play critical role in the inflammatory.Objective:To investigate the effect of the activation of microglia and the expression of TNF-α, IL-1βthe reperfusion after focal cerebral ischemia of rats by Naoxintong. Explore effective method to interfere the inflammation following cerebral ischemia-reperfusion injury.Methods:1. Thirty male SD rats were randomly divided into three groups:sham operated group(A group, n=10), Ischemic group (B group, n=10), Naoxintong group(C group, n=10).2. According to the instruction of Naoxintong, dissolve the medicamental pulverata of Naoxintong collocystis with distilled water. Take clinical medication of human×equivalent dose ratio of animal as reference concentration.0.14g/kg.d intragastric administration to C group for 2 weeks. A and B groups were intragastriced with equal distilled water. Took operation lhour after the last intragastric administration. 3. B and C groups were produced focal cerebra Ischemia-reperfusion by right middle cerebral artery occlusion with improved Zea-longe. Reperfusion 90mins later. A group expose common carotid artery and its crotch only, didn't block middle cerebral artery. All rats were execute 24hs after successful operation.4. The activation of microglia:CD68, IL-1βand TNF-αwere detected by immunohistochemistry and Western Blot.Results:1. Immunohistochemistory:the expression of CD68,IL-1βand TNF-αwere markedly increased in B group than those in A group; The expression of CD68,IL-1βand TNF-αwere significantly lower in C group than those in B group.2. Western blot:the expression of IL-1βand TNF-αwere markedly increased in B group than those in A group; The expression of IL-1βand TNF-αwere significantly lower in C group than those in B group.Conclusions:Naoxintong can inhibit inflammation reaction by inhibit the activation of microglia and the expression of TNF-α, IL-1βafter acute cerebral schemia-reperfusion injury, and which can provide protective effect on cerebral ischemic-repefusion injury in rats.Significance:This paper has made a further step on verifying that inflammation reaction is one of the most important characters of causing continuous injuries of the tissue while brain ischemia-reperfusion is happening, and almost menifested the relationship between brain acute ischemia and activation of microglia, also between brain acute ischemia and the release of inflammatory factors. It has verified that Naoxintong can have inhibition effects with inflammation reaction after cerebral ischemia-reperfusion, therefore provides new effective precautious measures for injuries of acute cerebral ischemia-reperfusion.