RASS System Antagonist Compound To SHR Big Mouse Cardiac Muscle Gap Junction Protein Cx43 Expression Intervention Research

IntroductionAt present, hypertension's disease incidence rate is getting higher and higher, the long-term hypertension not only has brought the pain to the patient, and has brought the serious economic burden for the society and the family, the long-term blood pressure will elevate will cause the heart, the brain, kidney and so on important internal organs' harm, the heart will be mainly exhausts oneself one of organs. Because the pressure load is overweight, will cause the ventricle on to remould for a long time with the cardiac muscle fibrosis, that if can suppress hypertension effectively to this stage transformation, means the treatment level the enhancement and medical expense reduction. The overseas scholar after the research discovered that the multi-cell life body's coordination behavior relies on the intercellular space connection and the communication, the neighboring cell clung the edge to constitute the intercalary plate, in the intercalary plate two cell's function contact areas namely for the intercellular space connection, it were one kind of special membrane protein structures, gathered by the intercellular space channel meets the formation, was the neighboring cell electricity and the chemistry pair joint foundation, was called the intercellular space connection communication. At present altogether discovered that 20 kind of connection proteins, the heart gap junction protein (connexin, Cx) mainly by Cx43, Cx40, Cx45 is composed. The Cx43 content is richest, mainly exists in working in the cardiac muscle. The Cx43 expression and the distribution exceptionally may cause a series of heart's change, for example:Congenital heart disease; Cardiac arrest; Arrythmia sudden death; The heart fades and the cardiac muscle is plump; Atherosclerosis. The long-term hypertension may cause Cx43 to express and the distribution reduction, then Cx43 and between gap junction's relations as well as the RASS system antagonist compound is not clear at present to its intervention mechanism, this experiment is the hope has the new breakthrough in this domain, for patient better choice voltage dropping medicine.Object and method1.Experimental animal groupingTo the animal model (12 weeks male gender, body weight 200 gram big mouse) carry on the grouping, divides into:The Wistar big mouse group, the SHR group, Reamey the Pulley group, for the Mischa Tanzania group, according to the Pulley alkone group, Reamey Pulley+for the Mischa Tanzania group, Reamey Pulley+ according to the Pulley alkone group, according to the Pulley alkone+for the Mischa Tanzania group, each group of 8, the freedom takes the food and absorbs the water, the compatibility feeds one week later starts to test, Reamey Pulley group 2.5mg/kg.d, for Mischa Tanzania group 10 mg/kg.d, mixes fills the stomach treatment according to Pulley the alkone 100 mg/kg.d in the few distilled water, the treatment course 8 weeks, every week measures the body weight, according to the body weight adjustment dose, the union medication takes half quantity.2. Measures the blood pressureBefore feeding,4,8 weeks measures the big mouse tail arterial pressure separately.3.Cx43 examination8 weeks later sudden dies the animal, takes out the heart, after latter heart organization paraffin wax embedding, makes the slice to observe organization's approximate shape and the myocardial cell trend, uses in the immunity histochemistry dyeing, observes Cx43 is unusual in the cardiac muscle organization's distribution and the expression.4.Statistics analysis Uses the SPSS11.0 statistical analysis system software to carry on the data analysis. Data were analyzed using one-way analysis of variance folloeed by a least significant test (LED) for multiple comparisons.P<0.05 is in statistics has the significance difference.Test result1. The big mouse blood pressure result determination demonstrated:The control depends on the Pulley alkone to the blood pressure with to unite the application for the Mischa Tanzania to be best, compares with other groups, has the remarkable difference (P<0.05), explanation aldosterone acceptor antagonist compound and blood vessel tense elementâ…¡.The acceptor antagonist compound's union application has the obvious control to the long-term blood pressure.2. The immunity group result showed:To Cx43 the intervention aspect, by with depends on the Pulley alkone union application effect for the Mischa Tanzania to be best.ConclusionTo big mouse's blood pressure intervention aspect, aldosterone acceptor antagonist compound and blood vessel tense elementâ…¡The acceptor antagonist compound's union application has the obvious control to the long-term blood pressure, its mechanism possible and these two medicine union application effective control main effect material aldosterone and blood vessel tense elementâ…¡Unifies with the acceptor, thus better display effect; But in suppressing the cardiac muscle fibrosis and to Cx43 the intervention aspect, also the unions effect is best.