Clinical Research On Inhibiting Capsule Opacification By 5-Fliuorouracil Into Capsule

Background and ObjectiveAfter-cataract estimates that after removal of cataract surgery, or after traumatic cataract, the len in the pupil appears len cortex, or formats fibrous membrane, which is also known as secondary cataract or posterior capsule opacification. In the early intraocular lens surgery stage when we ignored the the importance of clearing the len epithelial cells and the cortex, after-cataract was particularly widespread and serious. Anti-metabolism drugs have been playing an important role in inhibiting posterior capsular opacification. Among them, increased attention is devoted to 5-fluorouracil because of its high specificity, strong inhibition, and being less toxic to the eye. We base our experiment on a large number of detailed animal experiments and clinical trials home and abroad. During this experiment,5-fluorouracil was injected into the capsular bag by the home-made micro-injector,and then we observed its inhibitory effect on the after-cataract.This experiment will lay the groundwork for further investigation of after-cataract and provie the convenient and effective route of administration in clinical prevention and treatment.Materials and MethodsSubjects in the test were cataract patients who were in our department from March to September in 2009. The inclusion criteria was just age-related cataract patients, no other potential interference of test results,such as eye diseases and systemic diseases. We gave 107 eyes numbers, using random number table, randomly assigned to four groups, which were the experimental group A1, A2, A3 and the control group B. The experimental group A1, A2, A3 in the surgery using self-made micro-injector in the capsular bag were injected concentration of 0.25mg/ml,0.3mg/ml,0.35mg/ml of 5-fluorouracil 50μl. The control group was without this step. Experimental observation indicators include the Odrich scores on the first,third and sixth month during the post-operation, intraocular pressure,the observation of corneal endothelial cells,and /Subjects subjective feeling questionnaire/. Statistical analysis:Adoption of F and t tests,we analyzed all the materials through software SPSS 15.0.Results1. On the third month and the sixth month, the difference of Odrich scores between the test group and control group was statistically significant; At the same time, each Odrich score is positive, which means that 5-fluorouracil inhibitory effect on lens epithelial cell proliferation and migration is just limited.2. Between test group and control group, the difference between preoperative and postoperative intraocular pressure was not statistically significant.3. Between test group and control group, the difference between preoperative and postoperative corneal endothelial cells was not statistically significant.4. Between test group and control group, the difference bteween the positive rate postoperative discomfort and satisfaction rate of postoperative visual acuity was statistically significant.Conclusions1. By this micro-meter, injecting 5-fluorouracil into the capsular is simple, convenient and effective.2.5-fluorouracil on inhibition human lens epithelial cells is concentration dependent, while 5-fluorouracil eye toxicity is dose-dependent; 0.35mg/ml is just a balance between the two concentrations.3.5-fluorouracil just inhibits lens epithelial cells, and can not completely prevent the lens epithelial cell proliferation and migration.