The Concertrations And Significance Of Serum VEFG And ENA-78 In Patients With Endometriosis

Backgrounds and ObjectivesEndometriosis, which is defined as functioning of endometrial glandular epithelial cells and stromal cells growing somewhere else other than mocous membrane lining the uterine cavity, is a common diseases affecting 10-15% of the female population causing dysmenorrhea, infertility, pelvic pain,dyspareunia and so on. At present.The disease has brought a lot of confusions to the doctors of Obstetrics and Gynecology in diagnosis and treatment.Because its pathogenesis is not yet very clear, its gold standard for diagnosis is mainly from invasive laparoscopic examination and the rate of its recurrence is higher. In this study, the expression of VEGF (Vascular endothelial growth factor) and ENA-78 (epithelial neutrophil-activing peptide-78) in the serum of the patients with endometriosis are determined and the correlation between them is analyzed, in order to investigate the role of of VEGF and ENA-78 in the progression of endometriosis and obtain a guidance in the clinical diagnosis, treatment and prognosis of EMs. Furthermore, through researching on the above-mentioned factors'expression and regulation in endometriosis, a new method can be explored for the treatment of EMs by blocking formation of new blood vessels with the angiogenic factors as therapeutic targets. Materials and methodsRandomly selected the patients who have been laparoscopiced or laparotomied in Gynecology of the Second Affiliated Hospital of Zhengzhou Universiry from June 2008 to June 2009. The case group was patients whose pathology was diagnosed as endometriosis was diagnosed as after surgery.While the control group wes those pathology was diagnosed as uterine fibroids and the patients had laparoscopic examination or treatment for infertility over the same period. All patients'menstrual cycle were 28 days to 35 days and their fasting venous blood was taken before the operations. VEGF and ENA-78 concentrations were determined by ELISA.Database was established by Excel 2000 for VEGF and ENA-78 levels and all date were analyzed by SPSS13.0 statistical software,α<0.05 were considered to be significant.Results:①The expression levels of VEGF and ENA-78 in patients with EMs were significantly higher than those in the control group(P<0.05);②The expression levels of VEGF and ENA-78 in stageⅢandⅣwere significantly higher than those of stageⅠandⅡ(P<0.05);③The levels of serum VEGF were correlated positively with that of ENA-78 in women with EMs(rp=0.572 P<0.05);④The levels of serum VEGF and ENA-78 in patients with EMs were uncorrelated with the severity of dysmenorrhea(rp=0.043, rp=0.232,P>0.05;⑤Simultaneously testing the serum VEGF and ENA-78 levels for diagnosing endometriosis,the sensitivity was 56.3% and the specificity was 95.8%.Conclusions:1,The levels of VEGF and ENA-78 are correlated with the progression of endometriosis;2,The VEGF and ENA-78 levels are uncorrelated with the severity of dysmenorrhea;3,It may be valuable to detect the serum levels of VEGF and ENA-78 in diagnosing endometriosis.