| Backgrounds and ObjectiveTumor is the body's normal cells which start moving in a different long-term effects and the promotion of factors arising under the abnormal proliferation and differentiation of the formation of a new life, once formed continued to grow, disruption of normal tissues and organs. Malignant tumor threat to humanity more apparent, and has become one of the common causes of death. Malignant tumor in the primary site can not only grow invasively, involving the adjacent organs and tissues, but also can spread through a variety of ways to other parts of the body. This is the most important biological characteristics of malignant tumors, and also contribute to the cause of death in patients.Fibroblast activation protein (FAP) was reported as a membrane antigen in1988 by Retting, etc with the application of monoclonal antibody F19, belonging to serine protease class, with the ability of decomposing the gelatin and the activity of type I collagen and similar to 2 peptidyl-peptidase (dipeptidyl peptidase, DPP) activity, degradation of ECM, so that tumor cells from the original location is conducive to tumor cell invasion and distant metastasis. On the other hand, FAP may also be involved in bio-active peptides and chemokines in the cracking and thereby participate in tumor invasion and metastasis, and tissue remodeling. FAP antigen expression in a variety of malignant epithelial tumor stromal cells (such as breast cancer, ovarian cancer, lung cancer, colon cancer, esophagus cancer, etc.), while in normal tissues and benign tumors of fibroblasts positive expression of stroma-free, Tip it may be a proliferation of fibroblast surface markers, and malignant tumor growth, invasion, invasion.Transforming growth factor-β(TGFβ) is a dimer, the molecular weight of 25,000kD, consists of two 112-peptide chains through two disulfide bonds between the composition of the body's immune regulation, cell growth and differentiation, cell extracellular matrix synthesis and storage, embryonic development, wound repair, tumor immunity plays an important role. Mammals primarily contain TGFβ1, TGFP2, TGFβ3, of which, TGFβ1 is the major form of existence in the human body. In a variety of tumor tissues, TGFβ1 expresses over much and participate in tumor proliferation, differentiation, apoptosis and other pathological physiological processes, and can promote the reaction of tumor stroma formation. Thus, TGFβ1 may be related to the expression of FAP.In this study, immunohistochemical SP method was applied to detected FAP and the expression of TGFβ1 in lung adenocarcinoma to explore the expression intensity of both the inter-relationship, understanding both in lung cancer occurrence and development of the process of interaction analysis with lung clinicopathological parameters adenocarcinoma correlation between, for clinical diagnosis, treatment, prognosis and the development of comprehensive treatment programs provide a reference.Materials and MathordsFAP and TGFβ1 were detected by immunohistochemical SP method in 40 cases of paraffin-embedded tissue of lung adenocarcinoma cases. Both statistical analysis of expression in lung adenocarcinoma and its association with clinical and pathological features of the relationship between expression intensity of both at the same time to explore the inter-relationship. The other 10 patients taking chronic inflammatory lung bullae and lung tissue samples as controls. Statistical Package for all data handled by SPSS 17.0. With p= 0.05 level to test.Results1,FAP in the normal group, no positive expression in lung tissue, in 40 cases of adenocarcinoma of the lung tissue of the positive rate of 90%(36/40), mainly expressed in lung interstitial fibroblast cytoplasm, brownish yellow, had nothing to do with the pathological grade (p= 0.986), and clinical staging (p= 0.017), which increased with the clinical stage, FAP expression intensity increased. Some tumor cells also had a small amount of positive expression.2,TGFβ1 in normal lung tissues no positive expression in lung adenocarcinoma group, expressed in the cytoplasm of cancer cells, showing brown positive rate was 87.5%(35/40), and pathological grade and clinical stage were related (p= 0.030, p= 0.013), that, as the malignant degree of increase and the increase in clinical stage, TGFβ1 expression intensity had also increased.3,FAP and TGFβ1 expressions were positively correlated (r= 0.882, p= 0.000), that, as one factor expression enhanced the expression of another factor also increased.Conclusions1,FAP, as existed in a variety of epithelial stromal tumor of the factors in lung adenocarcinoma had positive expression of stroma, and was closely related to the proliferation of tumor invasion, suggesting that the situation could be used as indicators of tumor progression.2,TGFβ1 in lung adenocarcinoma was positive expression, with increased levels of malignant tumor cells increased expression of tumor cells related to the malignant degree, and the evolution of the degree associated with the tumor. Statistical analysis showed that FAP expression in a positive correlation had been in vitro studied confirmed the expression of TGFβ1 could promote the FAP.3,In lung adenocarcinoma, there is high expression of FAP and TGFβ1, and possibly through some molecular mechanisms,they promote proliferation and invasion of cancer cells, so that they can be considered as the degree of malignancy of lung adenocarcinoma and indicators of prognosis, and may be the target for lung cancer therapy. 4,Combined detection of FAP and TGFβ1 helped to determine the degree of malignancy of lung adenocarcinoma and estimation of prognosis for comprehensive treatment of lung cancer provide a useful help.
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