The Positive Effects Of Timetazidine Hydrochloride On Cardiac Muscle Cell After Acute Myocadial Infaction In Rats

Objective:Not only myocardial necrosis but myocyte apoptosis resulted from ischemia have been demonstrated in animal models as well as in patients with acute myocardial infarction. Trimetazidine, an antioxidantagent, has been extensively used in coronary artery disease and other cardiovascular disease. Also, it has been shown that trimetazidine may play a positive role in reliving the suffering of patient with angina pectoris and improving their living conditions. As a new type of anti-ischaemic drug, trimetazidine has good protection effects on human myocardium through inhibiting fatty acid oxidation, increasing glucose metabolism and improving energy metabolism in myocardial cells. In present study, we investigated the effect of trimetazidine on myocyte necrosis in animal models.Methods:44 Wistar White rabbits (half of these is male) were randomly divided into four groups. groupA:sham-operated group 8 (normal sodium group), group B:AMI group 12(AMI+normal sodium group), group C: treatment group1 12 [drug intervention (Trimetazidine)+AMI group], group D: treatment group2 12 [drug intervention (Simvastatin)+AMI group]. Group C were feed with Trimetazidine at the dosage of 30mg/kg/d. Group D were feed with Simvastatin at the dosage of 40mg/kg/d. Group A and B were feed with the same dose of normal sodium. All kept a period of 2 weeks. After 2 weeks of intragastric administration and 5 min after anesthesia, the heart was exposed after the chest had been opened. Group B,C and D was established into AMI models by ligating of the left anterior descending coronary artery but coronary artery wasn't ligated in the sham group.24h later Blood samples were taken from all groups. Hs-TnT level was assay with E12010. The pathological changes of myocardium were observed with microscope. The cardiac muscle samples were obtained to detect the expression of Bcl-2,BaxmRNA in noninfarcted zone by reserve transcription polymerase chain reaction(RT-PCR).Results:As compared with sham group, acute myocardial ischemia in AMI group induced significantly increasing in level of hs-TnT and in the expression of Bcl-2,BaxmRNA. The groups were treated with Trimetazidine or Simvastatin is significantly fewer in level of hs-TnT and in the expression of BaxmRNA than that in control group. But they are still more than that in sham-operated group. The expression of Bcl-2mRNA in treatment groups is more than that in AMI group. None of above three facts is markedly difference between the rabbits that were feed with Trimetazidine and Simvastatin.Conclusion:This experiment indicated that TMZ intervention have notable protective effect on cardiac muscle cell after Acute Myocardial Infaction. Pretreatment with trimetazidine may effectively prevent myocyte apoptosis from Acute Myocardial Infaction via mechanism of the increasing of the expression of Bcl-2mRNA and the decreasing of the expression of BaxmRNA.