The Expression And Clinical Significance Of The RUNX3 And P53 In Epithelial Ovarian Tumors

Background:Ovarian cancer is one of the three malignant tumors of female genital.It is thehighest death of cancer in the female reproductive system tumors,which accounted formost of epithelial ovarian cancer.Since the occult onset, no obvious early symptoms,about 70% of patients with advanced stage when treatment (which accounted for mostof epithelial ovarian cancer). Due to a lack of effective early detection methods inearly Ovarian cancer,which makes difficult to the early diagnosis. Thus, it is veryimportant to find its occurrence and development factors related for its early diagnosisand treatment,to provide effective solutions for improving the quality of life ofpatients RUNX3 is deeply involved in multiple cancer processes, such as cell growth,apoptosis, angiogenesis, metastasis, in various types of tissues. Identification of keytissue- and stage-specific target genes of RUNX3 functioning as a tumor suppressoror an oncogene is an important step forward, and combination of these genes wouldimprove the potential of usage of RUNX3 as a biomarker for cancer diagnosis. Duringthe course of tumor progression,RUNX3 protein has been showed to bedownregulated in the most cancers(eg, liver, stomach, colorectal, etc.). However,Nicole S. Nevadunsky et al inspected that RUNX3 protein is overexpressed in humanepithelial ovarian cancer,which can be seen the mechanism of RUNX3 is morecomplicated in the ovarian cancer. P53,the most frequently mutated gene in humanmalignancies. P53 activation leads to the upregulation of various target genesresponsible for cell cycle arrest or apoptotic cell death, depending on the cellularenvironment.Objective:To discusses the expression and relation of RUNX3 and P53, and analysis themechanism and clinical significance of RUNX3 and P53 in the epithelial ovariancancer process.Method:Immunohistochemical S-P was used to detect the expression of RUNX3 and P53in 20 cases of epithelial oothecomas tissue,20 cases of normal ovarian tissue,43 cases of epithelial ovarian cancers tissueResult:1,The expression of RUNX3 positive rate (79.1%) in epithelial ovarian cancerwas higher than in benign epithelial ovarian tumors and normal ovarian tissues(35.0%, 25.O%), the difference was significant (P <0.05). Serous carcinoma of thepositive expression rate (90.5%) than in mucinous carcinomas (50.0%) (P <0.05); Theexpression of RUNX3 positive rate in lymph node metastasis(95.5%) was higherthan those without lymph node metastasis (61.9%) (P <0.05); and RUNX3 expressionIs not related with age, clinical stage, histological grade.2,P53 protein positive rate (53.5% ) in epithelial ovarian cancer was higher thanin benign epithelial ovarian tumors and normal ovarian tissues (5.OO%, 10.00%), thedifference was significant (P <0.05); the positive rate (68.0%) of clinical stagingIII-IV than in Phase I-II (33.3%), the difference was significant (P <0.05); positiveexpression rate of P53 (82.4%) in histological grade 3 was significantly higher than1 ~ 2 (30.8%,38.5%) (P <0.05); with lymph node metastasis positive rate of P53(68%) .2than those without lymph node metastasis group (14.3%) (P <0.05); inpathology Subtypes, the positive expression rate (71.4%) in serous carcinoma thanin mucinous carcinomas (33.3%) (P <0.05); but not related with age.3,It is positive correlation between RUNX3 and P53 (P <0.05), while the higherRUNX3 expression, P53 expression is also higher.Conclusions:1,The study found that the expression of RUNX3 in epithelial ovarian cancerwas higher than that epithelial ovarian tumors and normal ovarian tissue, It can beseen in epithelial ovarian cancer RUNX3 might play a role in tumor oncogene,whileRUNX3 play a role in the invasion and metastasis of the malignant epithelial ovariantumors. It is showed that RUNX3 may be diagnosis, treatment and prognosis of a newway.2,The expression of P53 protein in ovarian cancer was higher than than inepithelial ovarian tumors and normal ovaries, and in poorly differentiated andadvanced ovarian cancer are highly expressed. P53 protein overexpression in ovariancancer biological is related with the behavior of malignant progression, may be usedas prognostic indicators of ovarian cancer. 3,This is the first explored the correlation between RUNX3 and P53 We foundthat RUNX3 and P53 in epithelial ovarian cancer have a positive correlation exists.