Brain-deried Neurotrophic Factor And Neuritin Genes The Expression And Meaning In Glioma

Objective:to study the specificity of intracranial glial tumor marker, and seeks to one or two specific expression in tumor cells to specific structure, tumor molecular diagnosis has special significance to tumor label Remember things.Especiallylookingfo BDN (brain-derivedneurotrophic) and Neuritin gene and intracranial glioma relations. Methods:using immunohistochemistry (Envision method) were measured in 80 cases of intracranial gliomas (includingⅠ,Ⅱ,Ⅲ,Ⅳlevel in 20 cases) each gliomas Neuritin gene and BDNF expression, and with 20 cases of normal brain tissue or high blood pressure as blank contrast,20 Edge brain cases of tumor weeks as controls. Results:(1) the brain and normal BDNF on high blood pressure of the brain and tumor weeks in expression rate small brain edge inⅢ, the expression of gliomas in supreme,Ⅰ,ⅡandⅢglioma, along with the rising of gliomas pathological grade rate of its expression is increasing gradually, expression and the strength and glioma, but positive correlation between pathological level in level IV glioma, its expression, even lower than the intensity suddenly reduce level-i gliomas, BDNF in tumor weeks organizations andⅠ,Ⅱ,Ⅲ,Ⅳlevel respectively of gliomas positive expression plus or minus9.11±1.11,24.76±0.45,49.67±2.22,84.34±0.89,18.78±1.65,, in normal brain tissue in no expression, each group has significant difference (P<0.01).The glial cells, BDNF inⅠ,Ⅱ, andⅢ, respectivelyⅣlevel of positive,50%,85%,25% between 20%, each group has significant differences (X2=35.13, P<0.01). Among them,Ⅲof gliomas, the expression of BDNF highest levelⅢgliomaⅠ,Ⅱlevel, higher than in classⅠ, gradeⅡ(r=0.6806, P<0.001), express intensity with gliomas pathological level increases. But in level IV glioma China expressed suddenly drops, between tumor weeks between organization and level I glioma. BDNF expression with patients age(X2=1.15,P>0.05 gender (X2=0.35,P>0.05), tumor size (X2 =0.230,,P>0.05) P, place irrelevant (X2=0.45,P>0.05). The cancer clinical factors and the expression of BDNF in gliomas no obvious correlation.(2) a Neuritin gene expression in tumor weeks in brain tissue, the edge ofⅠ,ⅡandⅢglioma of gliomas in expression rate increased with pathological level higher, but in level IV gliomas in expression suddenly drops, Neuritin genes in tumor weeks organizations andⅠ,Ⅱ,Ⅲ,Ⅳlevel respectively of gliomas positive 14.66±0.01,19.32±0.11,40.99±0.98,87.45±0.83,16.99±3.43, measured between, each group has significant difference (P<0.01). Among them,ⅢNeuritin protein expression of gliomas supreme,ⅢgliomaⅠ,Ⅱlevel, higher than in classⅠ, gradeⅡ(r=0.6977, p<0.001), express intensity with gliomas pathological level increases. Neuritin express respectively with the patients age (X2=0.03, P>0.05), gender (X2=1.91, P>0.05), tumor size (X2=2.34, P>0.05), place (X2=0.28, P>0.05), P is irrelevant. Neuritin genes to 20%,42%,90%,17% expressed in positiveⅠ,Ⅱ,Ⅲ,Ⅳlevel glioma, can obviously found their difference, diversity in each group has obvious statistical significance (X2=23.249, P<0.01).(3) Neuritin different degree of BDNF and expression. BDNF inⅠ,ⅡandⅢglioma is obviously higher than in expression significantly, normal brain tissue and levelⅣgliomas, hints of BDNF on tumor cells with early variation in content of gliomas closely related, the occurrence and development of BDNF expression of strength and early glioma, a positive correlation pathology classification that BDNF in gliomas in expression, the more that the malignant glioma, higher level, but at a certain degree of malignancy that after its expression, so BDNF can reduce for judging the glioma biological behaviour, forecasting patient outcomes, one of the important indexes of BDNF expression and age, sex, patient tumor size, site of the clinical factors such as not related, the patient age, gender, tumor size, parts and so on the clinical factors do not affect BDNF expression.Neuritin gene in theⅠ,ⅡandⅢglioma is obviously higher than in expression significantly, normal brain tissue and tumor weeks organization, the tumor cell Neuritin protein hints the content change and early development and occurrence of gliomas closely related, the Neuritin protein expression strength and early of gliomas pathologic stage a positive correlation, explain Neuritin protein expression in glioma, the more that the higher level of malignant glioma, also can serve as gliomas biological behaviour, forecasting judge the important index of patient outcomes. Neuritin protein expression and patient age, gender, tumor size, site not related, the patient age, gender, tumor size, site does not affect Neuritin protein expression. Both in early glioma positively correlated in expression, hints of gliomas Neuritin protein expression of BDNF and control mechanism, BDNF may exist in Neuritin protein by promoting the high gliomas expression makes the malignant glioma proliferation, but to levelⅣroom all lowers both expression may show right now is out of control and growing methods gliomas already no longer rely on cytokines, so further research Neuritin genes in BDNF, the expression and regulation mechanism gliomas, for the treatment of gliomas, can open a new method, provide new targets.