Expression And Relativity Research Of Cyclic B1 And Cyclic D1 In Severe Reflux Esophaguses, Barrett's Esophagus And Esophageal Adenocarcinoma

Objective:To investigate the expression of Cyclin B1 and Cyclin D1 in cases with severe reflux esophgitis, Barrett's esophagus, esophageal adenocarcinoma and the healthy controls, explore the role of Cyclin B1 and Cyclin D1 on the development of esophageal adenocarcinoma and evaluate whether these two cyclins could be bio-markers for risk of canceration of Barrett esophageal epithelia.Methods:72 patients in conformity with research condition were performed biopsy and pathological examination. They were grouped according to gastroscopy manifestation and pathological results, such as severe reflux esophagitis(RE,25 cases), Barrett esophagus(BE,35 cases), Barrett esophagus mixed with atypical dysplasia(DY,8 cases), esophageal adenocarcinoma (EA,12 cases). Ten cases with normal esophageal mucosa were examined as the control. Then patients with Barrett esophagus were sub-grouped according to Barrett esophageal length, mucous morphology and grades of intestinal metaplasia and atypical dysplasia. Expression of Cyclin B1 and Cyclin D1 in different groups were examined with immunohistochemistry and analyzed statistically.Results:Cyclin B1 and Cyclin D1 were high expression in the specimens of the BE and EA groups and very low expression in the control and RE groups. Statistical difference was showed (P<0.01).Expression of Cyclin D1 was increasing from the tissues of intestinal metaplasia, atypical dysplasia to adenocarcinoma (17.96vs 21.19 vs 32.63) and there was significant difference among these three groups(P<0.01). However no statistical difference was found about Cyclin B1 among the simple intestinal metaplasia(IM), Barrett's esophagus mixed with atypical dysplasia(DY) and EA groups (20.70vs26.31vs 29.88; P>0.05), but their average sum of ranks was increasing gradually. No difference about expression of Cyclin B1 and Cyclin D1 was found among Barrett esophagus with different lenths, mucous morphology of intestinal metaplasia and different grades of atypical dysplasia.Conclusion:Cyclin B1 and Cyclin D1 as markers of tumour development could evaluate the risk from Barrett esophagus to adenocarcinoma. Perhaps it is the early event in the development of esophageal carcinoma.