USE Of In Vitro Tissue Engineering Bone With BFGF Transfected In The REpair Of Segmental Radius Defects In The Rabbits

Objective To investigate the efficacy of combined use of CHA and bFGF transfected BMSCs for repairing segmental bony defects.Methods Tissue engineering bone was prepared and implanted in continuous defects in radius of adult rabbits, and Bioglass/BMSCs, autograft implantation or no graft were used as controls. The animals were sacrificed on 2,4,8 and 12 weeks after operation. The bone repair process was investigated using physical, roentgenographical, histological and biomechanical methods. X-ray observations, histological observed score results use software SPSS factorial analysis of variance, biomechanical test of variance analysis varied. Its mean differences have statistical significance. (P< 0.05)Results The defects in tissue engineering bone was repaired better than in Bioglass /BMSCs, autograft implantation or the control group 2,4 and 8 weeks after operation in histological and roentgenogram examination. The control group was filled wit fibrous and muscular tissues. Biologic mechanics showed that tissue engineering bone was growing more quickly and vigorously, the bone density was higher and the antibending force was stronger than autograft implantation after 12 weeks.Conclusion Tissue engineering bone with bFGF transfected has the biocompatibility, mechanical strength, the characteristics of source for rich, and has a bone formation, bone conduction and bone induction triple action.The treatment of bone defect poor blood supply of bone defect especially, and it has certain clinical use value.