Effects Of Hepatocyte Growth Factor On Cell Proliferation And The Expression Of Caspase-3 And TGF-β₁ In Human Cardiac Myocytes Cultured With High Glucose

Objective:To investigate the effects of hepatocyte growth factor (HGF) on the high glucose environment, human cardiac myocytes,HCM)proliferation and expression o f TGF-β1 and Caspase-3 in order to reveal the HGF in diabetic cardiomyopathy. in the protection of human cardiac myocytes Mechanism of action.Methods:①The human cardiac myocytes were divided into 3 groups in random: normal glucose group(5.5 mmol/L glucose),high glucose group(30mmol/L glucose) and high glucose plus hepatocyte growth factor group(30mmol/L glucose+25ng/ml HGF), After culture for 24 h,48 h,72 h, the proliferation activity of human cardiac myocytes was observed by MTT.②Enzyme-linked immunosorbent assay (ELISA) testing for normal glucose group, high glucose group , and high glucose plus hepatocyte growth factor( different concentration of hepatocyte growth factor : 25, 50, 100ng/ml,HGF group). The expressions of TGF-β1 and Caspase-3 were measured after culture for 48 h.Results:①The proliferation of human cardiac myocytes decreased in high glucose group (P<0.05), but increased in 25ng/ml hepatocyte growth factor group (P<0.05),and promoted time-dependently.②Normal glucose group, high glucose group , and high glucose plus hepatocyte growth factor groupafter culture for 48 h. Compared with normal glucose group, the expression of TGF-β1and caspase-3 of human cardiac myocytes in high glucose group was significantly increased, respectively (P< 0. 05). Compared with high glucose group, the expression of TGF-β1and caspase-3 of human cardiac myocytes in high glucoseplus hepatocyte growth factor group was significantly increased, respectively (P< 0. 05),and were suppressed dose-dependentlyConclusion: The continuing effect of high glucose could inhibit cell proliferation and inc- rease the expression of TGF-β1 and caspase-3,and promoted time-dependently hepatocyte growth factor could promote the proliferation activity and inhibit the epression of TGF-β1 and caspase-3 of human cardiac myocytes.The result prompts hepatocyte growth factor : maybe protect the diabetic heart by decreasing the epressions of TGF-β1 and caspase-3.