Comparative Study On The Effects Of The Rat AQP-5 Expression In Two Different Ways Of One-lung Ventilation

Objective To study the effect of two different ways of One-lung ventilation (OLV) on rat lung aquaporin protein-5 (AQP-5) expression.Methods The rats were divided into four groups. Group A: Performing OLV by clamping. Group B: Right bronchial intubation OLV. Group A and Group B was divided into A1, A2, A3, and B1, B2, B3 according to different ventilation time. There are two control groups. Group C: total lung ventilation group, which divided into C1, C2, C3 by the corresponding time with the experimental group. Group D: Vacuity contrast group. Take the lung tissue for Western blotting, observed the expression of AQP-5.Results The results of immunohistochemistry:Compared the expression of AQP-5, group A, B and C were less than group D (P<0.05), A, B, C compared within each group were significant differences (P<0.05); There is no significantly difference among the group A1, B1 and C1 (P>0.05); group A2 was less than group B2 and group C2 (P<0.05),and there is no significantly difference between B2 and C2; Group A3 and group B3 were less than group C3 (P<0.05),Group A3 was less than group B3 (P<0.05). The results of Western blot:Compared the expression of AQP-5, group A, B and C were less than group D (P<0.05), A, B, C compared within each group were significant differences (P<0.05); There is no significantly difference among the group A1, B1 and C1 (P>0.05) , and there is no significantly difference among the group A2, B2 and C3(P>0.05); Group A3 and group B3 were less than group C3 (P<0.05),Group A3 was less than group B3 (P<0.05).Conclusions Mechanical ventilation can reduce the expression of rat AQP-5, and time-dependent. Performing OLV by clamping and right bronchial intubation OLV have higher effect on the expression of rat AQP-5 than total lung ventilation, the former is more obvious.