| Objective To sieve and optimize prepare technology of Yiganzhuanyin granules. Methods The optimal extractive technology of Yiganzhuanyin granules was investigated using orthogonal design, and the effective component oleanolic acid and quercetin from Chinese medicinal herb were used as the quality control indexes. The method of prepared drug granules was determined. Results The optimal conditions for the extraction of Yiganzhuanyin granules was 10 fold amount of water, 3 times and 1.5 h each time. The ratio of dried extracts to diluents bases is dried extracts: starch: dextrin:sucrose=5:6:4:2. Conclusion The prepare technology is scientific and efficient. Objective:To establish a method for quality standard of Yiganzhuanyin granule.Methods:Spreading Hypericun japonicum Thunb.ex Murray and Herba Hedyotidis Diffusae were identified by TLC.The contents of oleanic acid and quercetin were determined by HPLC method. Hanbon Sci.&Tech. Dubhe C18 (4.6×250mm,5μm) was applied,The mobile phase were methanol-water- Phosphoric acid (45 : 55 : 0.4) and methanol-water(87:13),the determined wavelength were 360 nm and 212 nm. the flow rate was 1.0 ml·min-1,and the post temperature was 35℃.Results:Spreading Hypericun japonicum Thunb.ex Murray and Herba Hedyotidis Diffusae were identified successfully by TLC. The linnear range of oleanic acid was 0.054~4.320μg (R2=0.9994). The average recovery was 99.94%,and RSD was 1.00%.The linnear range of oleanic acid was 0.107~2.140μg(R2=0.9993). The average recovery was 100.46%,and RSD was 0.99%.Conclusions:The method is simple,sensitive and acurate . It can be used to control the quality of Yiganzhuanyin granule. Objective: To observe the protective effect of Yiganzhuanyin granule (YGZYG) on acute liver injury induced by acetaminophen(AP) in mice. Methods: The model of AP induced acute liver injury in mice with the method of subcutaneous injection were used to study the protective effects of YGZYG. Kunming mice were randomly divided into NC group, model group, high-, medium- and low- dose of UGZYG groups (0.4, 0.2 and 0.1 g/ kg respectively), and biphenyldicarboxylate (BPDC) group. The indexes of liver, spleen and thymus were observed. The activities of AST, ALT and ALP, the content of Alb, the ability of T-AOC in serum, the content of MDA, and the activity of SOD in liver tissue were investigated. Hematoxylineosin (HE) stain was used to examine the degree of hepatic injury. Results: Compared with model group, YGZYG could obviously reduce the weights of liver,spleen and thymus. The activities of AST, ALT and ALP, and the content of MDA could be decreased; the content of Alb, the ability of T-AOC, and the activity of SOD could be increased (P<0.01 or P<0.05). The degree of hepatic injury could be lessened. Conclusion: YGZYG has protective effect on acute liver injury induced by AP. The mechanism may be related to attenuating free radical and inhibiting the effect on lipid peroxidation. Objective: To observe the protective effect of LYQPS on immunity liver injury induced by concanavalin A in mice. Methods: 90 Kunming mice were injected with Con A (20mg/kg) via the tail at the first day of the experiment and then randomly divided into Con A group, high-, medium- and low- dose of LYQPS groups, and biphenyldicarboxylate (BPDC) group. 18 Kunming mice were used as NC group. All mice were administered with corresponding drugs by gastric perfusion at 8 hour after Con A administration. All the drugs were given once daily for 3 days consecutively. Four hours after the last administration of drugs, mice were injected with Con A once again at the same dosage. Blood samples for determining TNF-αconcentration were collected at 2 hour after Con A administration. The activities of AST, ALT and AKP in serum, the content of NO in hepatic microsome, the activity of SOD, the content of MDA and the expressions of Fas and FasL in liver tissue were investigated at 8 hour after Con A injection. Histopathological examination was also perfomed for liver tissue. Results: Compared with model group, LYQPS could obviously reduce the activities of AST, ALT and AKP, the contents of TNF-α, NO and MDA, and the expressions of Fas and FasL. The activity of SOD could be increased (P<0.01 or P<0.05). The pathological changes in liver tissue were lessened. Conclusion: LYQPS has protective effect on immunity liver injury induced by Con A. The mechanism may be related to down-regulation of TNF-α, antiapoptosis and inhibiting the lipid peroxidation.
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