Autofluorescence Spectroscopy For Human Colon Tissue And Its Endogenous Biomarkers

The clinical significances of detection for early colonic cancer, as well as the background of laser induced autofluorescence spectra technique applied in diagnosis for early colonic cancer was presented. The steady state and time-resolved autofluorescence spectra of human colonic tissues in vitro and its endogenous biomarkers were studied. Finally, the significant diagnostic parameters were also given, based on the algorithm of principal component analysis and two-peak raito.Firstly, a brief introduction to history of optical detection of early colonic cancer and the application status of laser induced autofluorescence technique in colonic cancer were reviewed. Furthermore, the protocol was presented based on fully preparation.Secondly, the properties of main endogenous biomarkers presented in human tissues were measured by FLS920 fluorospectrophotometer. In addition, a simple simulation of tissue optical model was developed.Thirdly, the characteristics of steady state and time-resolved autofluorescence spectra of normal and adenocarcinoma human colonic tissues in vitro were investigated. The obtained results indicate that the adenocarcinoma human colonic can be discriminated from the normal colonic tissue by using laser induced autofluorescence, and the optimal excitation wavelengths for the diagnosis of discrimination adenocarcinoma tissue were determined at about 280,340,400 and 460 nm. Moreover, under 337 nm excitation, some useful parameters were obtained based on PCA and "two-peak ratio" analyses.Finally, the obtained results in this study were summarized. Moreover, the potential studies were presented for further study.