| Chondroitin sulfate is an acidic polysaccharide. It mostly distributes cartilage, nose, throat, ligament and myolemma, especialy fish cartilage. CS has important physiological activity and pharmacological activities. CS is mostly used to treat neuralgia, arthritis, tinnitus, along with accelerating canker cicatrisation and preventing hyperlipemia. With lucubrating physiological action and biochemical character of CS, the effect of curor and preventing myocardial infarction is also found. CS can protect nerve cell, kindey cell. CS is also executed toiletry mixed with something to protect cutaneous beauty.With the new function and new USES expands nceasingly, chondroitin sulfate application scope is expanded. In the international market, chondroitin sulfate demand increase greatly in recent years. At present, the raw material of chondroitin sulfate is animals of cartilage, containing sharks, pig, ox, sheep, chicken, ducks, etc. Chondroitin sulfate has different content and kinds in different materials, and it has different clinical and business value. So it is important that identify chondroitin sulfate source of raw material. The method can improve the quality of chondroitin sulfate. It will be to make the contribution in the domestic market and the applied research.Near-infrared spectroscopy would be occurred when there were absorption due to overtone and combination bands originating from fundamental molecules vibrations correspond mainly to C-H,N-H and O-H groups in compound. Near infrared spectroscopy is a new and reliable quantitative and qualitative analysis method to organies, by using the chemometricsly tools to analyze the information showed in the NIR spectrum. The use of NIRS by the agriculture, chemical, food, bevarage and so on has grown steadily as the result of its advantages over conventional alternative. In the pharmic field, near infrared spectroscopy can be used for the quantitative and qualitative analysis, traditional Chinese drug quality, drug production quality on-line monitoring control, potions test, etc. General and main mode using NIRS is model-setting, model-verification, model-application and model-maintaining.Near infrared spectroscopy analysis is one of fast developing technology. Near infrared spectroseopic analytical technique is a "green" analytical technique. It has characteristics of simple, high efficiency, good precision, low cost, and convenient on-line analysis, etc.To review the reports concerning near infrared spectra in recent years, we summarized the research progress on authentication and content determination. We will establish a rapid method of identify chondroitin sulfate sources using near-infrared spectrum analysis technology advantages and different sources of chondroitin sulfate.First collected the spectrums of different sources from the same factory. To apply SIMCA, PLS-DA, SVMmethods, we establish identification model of three chondroitin sulfate sources. They are cattle, shark and pig. The recognition rate is 90%,100% and 100% separately in SIMCA with the pretreatment of 5 points smoothing in 10000 cm-1-4000 cm-1. The rejection rate is 40%,80% and 80% separately. The prediction ability of Model is lower. After first-order derivative and spectrum 5 point smoothing, we establish PLS-DA mathematical model in full-wave band. The mathematical model of forecasting ability is better than the SIMCA. After spectrum 9 point smoothing, we establish SVM mathematical model in the best condition. The cross-validation accuracy is 86.3636%.The predicted rate prediction set reached 93.3% correctly. Experimental results show that the SVM mathematical model has good prediction effect and high accuracy. It offers a new way for identify the sulfate sources.Fast qualitative analysis chondroitin sulfate sources without broken and using near infrared (NIR) spectroscopy were feasible. As the production or business enterprise to the identify chondroitin sulfate sources after this model corrected. |
