The Protective Effect Of Pinocembrin On Human Umbilical Vein Endothelial Cells Induced By Lipopolysaccharide

ObjectiveTo investigate the protective effect of pinocembrin on endothelial cells by a model of injured human umbilical vascular endothelial cells(HUVECs) induced in vitro by lipopolysaccharide(LPS),and explore the underlying mechanism, so to provide a new theory of flavone from propolis preventing atherosclerosis.Method1. In-vitro isolation and cultivation of HUVECs were performed using perfusion and digestion techniques.2. Pinocembrin from propolis was isolated by using column chromatography and it was elucidated on the basis of MS spectra.3. A typical inflammatory injury model of VEC was established by LPS.4. Cell-morphosis was observed by light microscope.5. Cells viability was assessed by MTT assay.6. Production of vWF were measured by ELISA.7. HUVECs apoptotic rate was detected by TUNEL.Results1. Effect of pinocembrin on viability of HUVECs Viability of HUVECs in injured group is significantly lower than that in control group, but increased significantly after interference of pinocembrin with 100 mg/L and 200mg/L (p<0.01). The effect is time-and-dose dependant.2. Effect of pinocembrin on production of vWF Production of vWF in injured group is significantly higher than that in control group, but decreased significantly after interference of pinocembrin with different concentrations (p<0.01). The effect is time-and-dose dependant. 3. Effect of pinocembrin on Apoptosis of HUVECs Apoptosis index in injured group is significantly higher than that in control group, but decreased significantly after interference of pinocembrin with different concentrations (p<0.01). The effect is time-and-dose dependant.4. Effect of combination of pinocembrin and simvastatin on HUVECs Compared to single administration, combination of pinocembrin and simvastatin had significant better effect on apoptosis index and secretion of vWF(p<0.01).Conclusion1. In injured HUVECs model induced by LPS, the apoptosis and secretion of von Willebrand factor increased,and viability of HUVECs decreased significantly.2. Pinocembrin may be hopeful for the protection of vascular endothelial cells by inhibiting apoptosis and the secretion of vWF and increasing viability of cells, showing a time-and dose-dependant manner.3. The protection of pinocembrin on endothelial cells may be synergic with inhibitor of HMG-CoA reductase such as simvastatin sodium.