The Protection Of Total Saponins Of Achyranthes Bidentata In Myocardial Ischemic Injury Of Rats

Objective:To observe the effect and the mechanism of ABS on acute my-ocardial isehemia rat.Method:To divide the 72 rats into control group, model group, low dosage gr ou p (0. 75g/kg), middle dosage group(1.0g/kg), high dosage group(1.25g/kg), FufangDansh-enDiwan group. With the way of venous injection of pituitrin below the rats'tail, to establish the acute myocardial ischemia. To observe the change on the electrocardi-ogram and CK,LDH,SOD,MDA to study the success of model and the effect of ABS on the acute myocardial ischemia. And to observe the change on the content of cTnT,ET,NO, TXB2 and 6-Keto-PGF1αto study the efficacy and mechanism of ABS.Result:Compared with nomal group,model group can definitely affect the electrocar-diogram with the changing of△ST,HR and P-R interphase and increase the CK,LDH and cTnT.Compared with model group,ABS high-dose group,middle-dose group low-dose group and CDDP group can obviously affect the increasing of△ST and P-R interphase and the decreasing of HR.And the amount of LDH,CK and cTnT of ABS group and CDDP group was lower than model group(P<0.01,P<0.05).These results suggested that The ABS is capable of improving the effect caused by the myocardial ischemia on rats after experiment of pituitr.Compared with model group,ABS high-dose group,middle-dose group low-dose group and CDDP group SOD contents were more than model group,and the MDA contents were obviously lower than model group(P<0.01,P<0.05),indicated that the ABS can suppressed the drop of total anti-oxidative capacity.Compared with model group,ABS high-dose group,middle-dose group and lower-dose group NO contents were more than model group(P<0.01),and ET contents were lower than model group(P<0.01,P<0.05),indicated that ABS had a certain effect on vascular endothelial cells.Compared with model group,ABS high-dose group,middle-dose group and low-dose group TXB2 contents were lower than model group(P<0.01,P<0.05),and ABS high-dose group and middle-dose group 6-Keto-PGF1αa contents were more than model group(P<0.05),indicate than it can increase the ratio of PGI2/TXA2 in the myocardial ischemia on rats.Conclusion:1. With the way of venous injection of pituitrin below the rats'tail, model group can definitely affect the electrocardiogram with the changing of△ST,HR and P-R interp-hase,indicated that the model is successful.2.The results indicated that ABS is capable of improving the effect caused by the myocardial ischemia on rats after experiment of pituitr.And the ABS could improve the electrocardiogram,decrease the release of enzyme and cTnT of myocardial.3.The ABS could decrease thecontents of MDA and increase the contents of SOD.It indicated that ABS has function on anti-lipid peroxidation.4.The ABS could decrease the contents of ET and increase the contents of NO.Tt indicated that ABS has effect on dilating vessels and protecting endothedial.5.The ABS could decrease the contents of TXB2 and increase the contents of 6-Keto-PGF1α.It suggested that ABS has important action on function of vessels,and it also inhibit platelet aggregation and thrombus formation.