| ObjectiveTo explore the cause of osteogenesis imperfecta (OI) bone fragility by the research on differences in pyridinoline cross-link formation at the N-and C-termini in typeâ… collagen molecules in urine from OI and healthy control children,which were age and gender-matched with the form.Methods20 children with OI and 20 nomal control subjects without OI matching with age and gender were detected. Some information such as their age and gender was collected,some indicators for serum bone alkaline phosphatase (BALP), serum Ca, serum P, parathyroid hormone (PTH), urinary N-terminal Telopeptides of Typeâ… collagen (uNTx), urinary C-terminal Telopeptides of Type I collagen(uCTx), urinary hydroxyproline(uHyp) and urine creatinine (uCr) were determinated. Method for enzyme-linked immunosorbent assay (Enzyme-Linked Immunosorbent Assay, ELISA) was applied. The data were analyzed with SPSS for Windows 13.0 statistic sotfware and Presented by mean(X) and standard deviation(SD).The Student-Newman-Keuls and the independent-samPle t test were used to compare groups.The relationships between the dieffrent variables were caleulated by calculating Pearson correlation coeffieients.Results1 Age and serum biochemical test resultsAge and gender between the observation or/and control groups were no significant difference, serum bone alkaline phosphatase, serum Ca, serum P, parathyroid hormone and biochemical test results between the observation or/and control groups were no significant, either.2 Typeâ… collagen cross-linked N-terminal peptide and typeâ… collagen cross-linked C-terminal peptide test results in urineIn urine of patients in the observation group typeâ… collagen cross-linked N-terminal peptides and C-terminal peptide and is closely related to age (r=-0.534 P=0.03,r=-0.528 P =0.01),normal subjects also (r=-0.486 P=0.03, r=-0.623 P=0.003), urine of patients in observation group typeâ… collagen in the cross-linked N-terminal peptide and C-terminal peptide lower than that of the control group. In the observation group typeâ… collagen cross-linked N-terminal peptide level OI typeâ…¢in the urine of was lower than that of OI typeâ… and typeâ…£, while OI typeâ… andâ…£was no significant difference. In the observation group,urinary typeâ… collagen cross-linked C-terminal peptide levels showed no statistical difference between OI typeâ… ,,â…¢andâ…£.3 The ratio of Urinary typeâ… collagen cross-linked N-terminal peptide or C-terminal peptide and urinary hydroxyproline, respectivelyThe ratio is used to analyze the ratio of uNTx and u CTx in collagen metabolism of the human body. Urinary hydroxyproline is no correlation with OI type, but with age (OIr=-0.734 P=0.000 healthy children, r=0.585 P=0.007). In the normal control group, the ratio of uNTx or uCTx and urinary hydroxyproline was no correlation with age respectively. The ratio of uNTx or uCTx and urinary hydroxyproline of the observation group was lower than that of healthy contron children. The ratio of uNTc and urinary hydroxyproline of OI typeâ…¢was seriously lower than that ofâ… and typeâ…£,â… and typeâ…£no difference. The ratio of uCTx and urinary hydroxyproline of sub-OI was no significant difference.Conclusions1 The uNTx and uCTx content of OI patients less than that of normal children., and typeâ… collagen N-terminal cross-linked structure of pyridine Nohrin decrease.2 The difference of this structure is related to severity of OI. The uNTx and uCTx can be a reference index, which assessed the severity of OI clinically, and there is a important significance in clinic.3 This research indicates the bone resorption markers value in the diagosis and treatment of OI, and provides new idea and throad. |
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