Expression And Significance Of MUC2 And CD24 In Ovarian Epithelial Carcinoma

Background and ObjectiveOvarian cancer is one of the most common gynecologic malignant tumors. Because ovarian cancer spot locates in the deep pelvic bottom absencing early symptoms and signs, early diagnosis difficult, time of diagnosis in 75%-80% patients with already advanced, the case-fatality rate highest in the first gynecological malignancies,5-year survival rate at only 35%. One of the biological characteristics of malignant tumour is to soak and shift.Ovarian is the largest and the most transfer site of tumors, early detection of metastatic ovarian cancer is not easy to difficult to treat is known,.In the metastatic ovarian cancer, gastric adenocarcinoma and colon adenocarcinoma are the most common cancer.Explore the mechanism of invasion and metastasis of ovarian cancer and looking for can predict the transfer and biological indicators to guide clinical treatment of ovarian cancer has become the research hotspot. In recent years, the functions of cell adhesion molecules and with the relationship between ovarian cancer researcher has become the focus of attention.Cell adhesion molecules are a class of glycoproteins, that can be expressed in a variety of cell, and involved in cell-cell and between cells and extracellular matrix of mutual recognition and interaction. Cell adhesion molecules play an important role in the process of tumor invasion and metastasis. Cell adhesion molecules can be roughly divided into six categories, including selectin family and Mucins family. Mucin is a group created by a different gene encoding a high degree of glycosylation and high molecular weight glycoproteins, which is divided into membrane-bound mucin and secreted mucin.Mucin synthesis by the epithelial cells.Under normal circumstances, Mucin play a protecting and lubricating role on the epithelial cells.Mucin2 is a secretory mucin and usually considered to be a gut-associated antigen, that normally expressed mainly in the colon and rectum.Mucin2 may be involved in cell-cell recognition and adhesion. Mucin involved in tumor metastasis, that through enhanced adhesion tumor cells to normal cells and reduce the adhesion to between tumor cells and in the tumor cell surface by steric hindrance phenomenon that tumor cells to escape immune recognition. Over-expression of MUC2 with a variety of malignant tumors related to the occurrence. Cluster of differentiation 24 is the major ligand of P-selectin, which is a low molecular and highly glycosylated cell adhesion molecules, which is linked to the cell membrance surface via a glycosylphosphatidylinositol anchor. CD24 expression in tumors in a variety of systems involved in tumor cell proliferation, differentiation and apoptosis.CD24 with the corresponding P-selectin ligand binding that mediated adhesion between cells and the proliferation of tumor cells transfer process.CD24 is considered as a new cancer-causing genes and metastasis-enhancing factor.Ovarian cancer mortality,5-year survival rate,poor prognosis and factors related to the gynecologic oncology researchers has been the focus of attention. Prognostic factors for ovarian cancer before from different studies are failing to carry out the purpose of this study in terms of adhesion molecules from primary and metastatic ovarian cancer development and metastasis research. MUC2 has been reported at home and abroad and high CD24 expression in ovarian cancer znd the prognosis of patients,but aslo detected in ovarian cancer has been peported rarely.In this study, the expression of MUC2 and CD24 protein in the primary epithelial ovarian cancer and digestive tract of metastatic ovarian cancer were tested by immunohistochemical method, and observe the kinds of proteins and the relationship between epithelial ovarian cancer in the clinical and pathological parameters and the correlation between the two. Materials and Methods1.83 cases of primary ovarian tumor tissue,10 cases of normal ovarian tissues,9 cases of colon metastasis of ovarian tissues and 10 cases of gastric cancer metastasis of ovarian tissue were collected from department of gynaecology and obstetrics in the third hospital affliated to Zhengzhou University between Jan 2003 and November 2008. All samples were diagnosed by two pathologists and all patients had not accepted the radiotherapy and chemotherapy before operation with complete clinical and pathological information.2.83 cases of primary ovarian epithelial tumors,including 16 cases of benigntum-or,19 cases of borderline,48 cases of malignant epithelial carcinma(26 cases of sero-us,22 cases of mucinous).Epithelial ovarian cancer according to FIGO (2000 years) clinical stage:17 cases ofⅠ,Ⅱperiod,31 cases ofⅢ,Ⅳperiod;histologic grade:20 cases of G1 Grade,17 cases of G2,11 cases of G3 Grade.There were 28 cases of omental transfer without omentum metastasis,20 cases;27 cases with lymph node metastasis,no lympy node metastasis in 21 cases.3. Using immunohistochemical SP method detected the MUC2 and CD24 protein expression in 83 cases of primary epithelial ovarian tumors,10 cases of normal ovarian tissue,10 cases of gastric cancer metastatic ovarian tissue and 9 cases of colon cancer metastatic ovarian tissue, and analysis of MUC2,CD24 protein expression and various clinical pathological data of the various inter-relatio-nships.4. The data were analyzed by statistics software SPSS13.0 package.Multi-samples rate comparison used contingency table chi-square test, rank-sum test and two sampiles rate used fourfold table chi-square test. Spaearman rank correlation analysis was used to analyze the relevance.The test standard was a=0.05.P<0.05 statistically significant.Results1. MUC2 and CD24 the positive expression rate of the order increaed in epithelial ovarian tumor tissue, respectively,benignn 25.00%(4/16) and 12.50%(2/16), borderline 26.32%(5/19) and 52.63%(10/19),malignant 66.67%(32/48) and 83.33%(40/48). Both in normal ovarian tissue are positive expression rate of O.The overall difference was statistically significant (MUC2:χ2=13.586; CD24:χ2=26.788, P<0.05);MUC2 and CD24 protein in epithelial ovarian cancer tissues was significantly higher than benign epithelial tumor tissue, the difference was statistically significant.2. MUC2 and CD24 protein in PhaseⅢⅣexpression was significantly higher thanⅠⅡstage tissue in epithelial ovarian cancer tissue,the difference was statistically significant(MUC2:χ2=4.554, CD24:χ2=6.576, P<0.05);Two kinds of proteins with lymph node metastasis of the cancer tissues was significantly higher than without lymph node metastasis of carcinoma,the diffrence was statistically significant(MUC2:χ2=6.095, CD24:χ2=7.467, P<0.05); MUC2 in mucinous tumor tissues was significantly higher than serous tumor tissues,and the difference was statistically significant(χ2=10.741,P<0.05);CD24 omentum metastasis in a cancer tissues than in those without omentum metastasis cancer tissues was significally(χ2=6.575,P<0.05);MUC2 and CD24 expression with histological grade and patient s age has nothing to do,MUC2 expression and omentum metastasis,CD24 expression has nothing to do with the histological classification(P> 0.05).3. MUC2 in colon cancer metastasis of ovarian tissues expression was significantly higher than primary ovarian carcinoma (χ2=4.171,P< 0.05),Gastric cancer metasis in ovarian carcinoma and primary ovarian cancer tissues no significant differences (P>0.05); CD24 in metastatic ovarian cancer and in primary ovarian cancer in the expression of difference was not statistically significant (P> 0.05).4 CD24 in epithelial ovarian cancer is higher than in the postive expression rate of MUC2 protein,and both the same tendency to compare the two in primary ovarian cancer tissues were positively correlated (r= 0.461, P< 0.05).Conclusion1. MUC2, and CD24 proteins in ovarian benign, borderline and malignant epitheli-al tumors of the positive expression rate of turn increased, and in epithelial cancer in high expression,and the degree of malignancy of epithelial carcinima, clinical stage and lymph node metastasis;MUC2 and histological classifications,CD24 is also associated with the transfer of greater omentum.show tha the both the occurrence of epithelial ovarian tumor development and metastasis may have a role.2. MUC2 colon cancer metastasis in ovarian tissues was significantly higher than primary ovarian carcinoma (P< 0.05), prompted MUC2 could serve as a source of ovarian metastatic colon cancer diagnostic marker for clinical use. 3. MUC2 and CD24 in primary ovarian cancer tissues were positively correlated (r = 0.461, P< 0.05), speculated that both the in epithelial ovarian cancer in the genesis and development and metastasis has a synergistic effect,but its specific role in the mechanism remains to be further studied.