The Expression And Location Of NIS In Differentiated Thyroid Carcinoma And Corresponding Lymph Nodes Metastasis

Objective: To study the gene expression of sodium/iodide symporter (NIS) in both differentiated thyroid carcinoma (DTC) and correlated lymph nodes metastasis. To explore the relation ship between NIS and DTC,and the significance of radioactive iodine treatment for DTC. Methods: The expression of NIS in tissue specimens was evaluated by RT-PCR technique and immunohistochemistry. Results: real time polymerase chain reaction (RT-PCR): T The expression of NIS mRNA didn't show significant difference between adjacent tissues group and DTC group. The NIS mRNA expression in lymph nodes group were lower compared with other groups .Immunohistochemistry: Compared with normal thyroid, NIS protein positive ratio in DTC and correlated lymph nodes metastasis tissue didn't show significant difference, respectively. Conclusion: The NIS could be normally expressed , but NIS protein was mostly expressed in the cytoplasm rather than in the functional membrane, which has clarifying the expression of NIS in DTC, but the iodine-uptake capability should be a further research.