The Effects Of Formoterol And ICI118551 On The Proliferation And Differention Of Rat Osteoblast-like Cells Induced From Marrow-derived Mesenchymal Stem Cells In Vitro

【Objectives】:To evaluate the effects of Formoterol(β2-adrenergic receptor-specific agonist) and ICI118551(β2-adrenergic receptor-specific antagonist) on rat osteoblast- like cells induced from marrow-derived mesenchymal stem cells(MMSC) .【Methods】:MMSCs by whole bone marrow adherent culture derived from a three-week-old female SD rat are induced to osteoblast-like cells.After different concentrations of Formoterol and ICI118551 are added into the culture medium, the alkali phosphatase is detected by PNPP and the osteocalcin is detected by radio-immunity.The cell proliferation is evaluated with the method of MTT.The cell apoptosis is evaluated by the methods of Annexin V-FITC/PI and Tunel.【Results】: The alkali phosphatase,osteocalcin and proliferation of cells cultured with all different concentrations(10-5-10-9 mol/L) of Formoterol are obviously lower that of the control.As to ICI118551, high concentrations(10-5 and 10-6 mol/L) can inhibit the alkali phosphatase, osteocalcin and proliferation of cells while low concentrations (10-8 and 10-9mol/L) can enhance the alkali phosphatase,osteocalcin and proliferation.Both Formoterol and ICI118551 have minimal effects on the cell apoptosis.【Conclusions】: Theβ2-adrenergic receptor-specific agonist Formoterol can inhibit the activity of rat osteoblast-like cells in vitro while the effects of theβ2-adrenergic receptor-specific antagonist ICI118551 are not single, depending on its concentration.Both of them have minimal effects on the cell apoptosis.