ESR Study Of Antioxidation By 10 Kinds Of Extracts From Chinese Herbs

Oxygen free radicals (OFR) also known as reactive oxygen species, including superoxide anion radical (O2·), hydroxyl radical (OH·), hydrogen peroxide (H2O2), singlet oxygen (1O2·), lipid oxygen free radicals (RO·), lipid peroxy radicals (ROO·), NO and so on. Oxygen free radicals are closely related with many diseases. Therefore, what people have been looking forward to solving is preventing or reducing the body's oxygen free radicals. Although the traditional synthetic antioxidants have strong antioxidant capacity, they also have long-term of potentially toxic, some even inducing teratogenic and carcinogenic effect. Thus finding natural, efficient, low toxicity anti-oxidants from Chinese herbs has become an inevitable trend. Relevant experiments in vivo and vitro showed the 10 kinds of chinese herbs including Scutellaria baicalensis Georgi,Carthamus tinctorius L., Camellia sinensis(L.)O.Ktze,Hypericum perforatum L.,Sophora japonica L., Oroxylum indicum (L.) Vent., Fagopyrum dibotrys(D.Don), Lonicera japonica Thunb., leaves and fruits of Crataegus pinnatifida Bge. have antioxidant activity by different degrees.Therefore, this study was to investigate the effect of 10 kinds of extracts from Chinese Herbs on the oxidative injury in order to provide the scientific evidence for wide clinical application. 1. Antioxidant effects of water extracts of Scutellaria and Carthamus tinctorius in senescence accelerated mouse(SAM-P/8)Antioxidant effects of aqueous extract of Scutellaria and Carthamus tinctorius were observed by selecting senescence accelerated mouse(SAM-P/8). SAM-P/8 were randomly divided into seven groups:control group with saline only, aqueous extract of Scutellaria dose groups (7.6,3.8,1.9 g crude drug g·kg-1)and aqueous extract of Carthamus tinctorius dose groups (7.6,3.8,1.9 g crude drug g·kg-1), which had been consecutively received by intragastric administration for 4 weeks. Thymus and brain were collected to weigh and calculate organ coefficient.The activities of superoxide dismutase (SOD),glutathione peroxidase(GSH-Px), catalase(CAT) and the contents of malondialdehyde(MDA) in serum and brain homogenate of all groups were determined by kit. The results demonstrated that they could elevate the brain and thymus coefficient. They can also improve the activities of SOD, CAT, GSH-Px in different degree and decrease the contents of MDA.2. The effects of 10 kinds of ethanol extracts from Chinese Herbs on Fe2+-ADP-NADPH induced lipid peroxidation(LPO) in rat liver microsomesThe effects of 10 kinds of ethanol extracts from Chinese Herbs on Fe2+-ADP-NADPH induced LPO were evaluated by calculating IC50 which repsents the concentration for 50% inhibition of LPO. The results demonstrated that ethanol extracts from Chinese Herbs and Trolox could significantly inhibit the generation of MDA induced by Fe2+-ADP-NADPH oxidation system and presented dose-response relationship in a certain range of concentration. IC50 of Trolox, Scutellaria baicalensis Georgi,Carthamus tinctorius L.,Camellia sinensis(L.)O.Ktze, Hypericum perforatum L., cold leaching of Sophora japonica L.,refluxing of Sophora japonica L. Oroxylum indicum (L.) Vent., Fagopyrum dibotrys(D.Don), Lonicera japonica Thunb., leaves and fruits of Crataegus pinnatifida Bge. are 0.014,0.066,0.598,0.030,0.041,0.041,0.058,0.098,0.324,0.458,1.069,6.156 mg·mL-1.3. Antioxidant effects of 10 kinds of ethanol extracts from Chinese Herbs on ox-LDL induced injury in cardiac microvascular endothelial cells The protection of 10 kinds of ethanol extracts from Chinese Herbs and Trolox were investigated after ox-LDL (100μg·mL-1) induced injury on the third generation of rat cardiac microvascular endothelial cells (rCMEC). The supernatant was collected for detecting MDA, SOD, GSH-Px activity. The results demonstrated that 10 kinds of ethanol extracts from Chinese Herbs increased the rCMEC survival rate, reduced MDA contents and improved SOD, GSH-Px activity.4. The effects of 10 kinds of ethanol extracts from Chinese Herbs on xanthine-xanthine oxidase system injury in cultured neonatal rat cardiomyocytesAntioxidant effects of 10 kinds of ethanol extracts from Chinese Herbs and Trolox were studied in the cultured neonatal rat cardiomyocytes which were injured by xanthine-xanthine oxidase system injury. The supernatant was collected for detecting lactate dehydrogenase(LDH), MDA, SOD, GSH-Px activity. The results demonstrated that 10 kinds of ethanol extracts from Chinese Herbs and Trolox increased the cardiomyocytes survival rate, and reduced LDH, MDA levels, and improved SOD, GSH-Px activity.5. The effects of ethanol extracts from Camellia sinensis (L.)O.Ktze, cold leaching of Sophora japonica L., Oroxylum indicum (L.) Vent. on oxygen derived free radicals (OFR) signal of cardiac microvascular endothelial cells injured by ox-LDL and cardi-omyocytes injured by xanthine-xanthine oxidase systemAntioxidant effects of ethanol extracts from Camellia sinensis (L.)O.Ktze, cold leaching of Sophora japonica L., Oroxylum indicum (L.) Vent. on OFR signal were investigated by selecting cardiac microvascular endothelial cells injured by ox-LDL and cardiomyocytes injured by xanthine-xanthine oxidase system. cell suspension was collected for detecting OFR singal by electron spin resonance (ESR). The results demonstrated that OFR signal of the cell suspension decreased significantly.Therefore, the results were suggested that 10 kinds of extracts from Chinese Herbs had anti-oxidation. The mechanism may be related with scavenging of free radicals, enhancing its clearance, enhancing endogenous antioxidant activity.