The Protective Effects Of Rosiglitazone On Lung Ischemia -reperfusion Injury In Rats

Objective To design a rat model of lung ischemia-reperfusion injury, to investigate the practical changes of the lung histopathology and determine the W/D of the lung,to determine the activity of MDA and TNF-αin abdominal aorta blood ,and to determine the expression of MPO and CINC-1 in the lung tissue by using SD rats, to approach the influence and mechanism of action in rat lung ischemia-reperfusion injury by rosiglitazone that is a PPARγligand.Methods Experiment were randomly divided into four groups,①Sham- group(A group)(n=5 ): Namely, given mechanical ventilation after thoracotomy 4h,the left pulmonary hilum was dissociated after thoracotomy without occlusion and mechanically ventilated to the end .②Sham operated and rosiglitazone group (B group)(n = 5),SD rats were ROS ( 0.3 mg / kg) by intravenous injection in 2 hours before surgery and given mechanical ventilation after thoracotomy 4hours ,the left pulmonary hilum was dissociated after thoracotomy without occlusion and mechanically ventilated to the end.③Ischemia Reperfusion group(C group)(n= 7): the left pulmonary hilum was occluded for 45minutes after thoracotomy and reperfused 3 hours,④Ischemia Reperfusion and rosiglitazone group(D group)(n= 7) : SD rats were given ROS ( 0.3 mg / kg) by intraperitoneal injection in 2 hours before surgery,and the other steps were same as the Ischemia Reperfusion group.Results compared to the D group, the lung wet-to-dry weight and the expression of TNF-a,MPO,MDA,CINC-1 in lung of C group rats were significantly elevated (p<0.01); Comparing with B group, there are not apparent statistic variances in A groups. In the aspect of pathological changes the alveolar and interstitial lung leukocyte were aggregated into a group the alveolar structural were damaged the alveolar interval turned wider,the alveolar space appeared severe edema and bleeding While pathological changes were slight in D groups. Conclusion Rosiglitazone significantly reduced ischemia-reperfusion injury in rat lung inflammation,and played a protective role on rat lung ischemia-reperfusion injury.