| Objective : 1. To observe the uptake of 18F-FLT in vitro of lung adenocarcinoma SPC-A1cell which in the different situations of division cycle and to correlate that relationship with the cell proliferation of the tumor. 2. To evaluate the use of 18F- FLT PET/CT for the diagnosis of non-small cell lung cancer.Methods:1.The cell proliferation was blocked by drug intervention. Respectively, we harvested the G2/M phase, S phase, G0/G1 phase-based sample for each batch, a total of three batches of 36 samples. There were about 5×106 cells in each culture bottle. 1.85×105Bq(5μCi)18F-FLT was added to each bottle and continue incubated for 60 minutes, washed by PBS for two times, and then digested with 0.25% trypsin and adherent cells were collect to get single cell suspension fluid. That were divided into two parts, for one parts of cells, detected the synchronization ratio by FCM after a 24-hour periodsa,and expressed as percentage in each cycle. For another part of cells, counted theγ-counts .In order to exclude the impact of the batch starting measured time for different pairs of count rate, each time set a 1.85×105Bq 18F-FLT standard pipe at the same time, the uptake rate take as the percentage of the radioactivity counts per 106 cells with the counts of standard pipe. 2.In clinical study,the 20 patients, of the same period with18F-FDG and 18F-FLT imaging were detected from Dec 2007 to Dec 2009, of which 12 men and 8 women, 30 to 88 years old, mean age of 61.33±16.94 years. Each 18F-FDG and 18F-FLT imaging were carried out under the same conditions. Every patient got definite diagnosis from pathological analysis or close follow-up. To carry on vision analyze and half quantitative analysis separately as PET result of phannaeeutieal,among them FDG standard adopted malignant SUV>2.5,FLT standard adopted malignant SUV>1.35.Results :1.The 18F-FLT uptake of every 1×106 lung adenocarcinoma SPC-A1 cells were significantly correlated with the cell percentage of S phase cells (r = 0.642, P <0.01), and also significantly correlated with the cell percentage of G2/M phase cells (r = 0.695, P = 0.001). 2. The 18F-FLT uptake of S-phase cells is higher than G2/M phase cells (P<0.05). 3. Of the 20 cases, 18 cases were solitary lung shadow or nodules, 2 cases were 2 lesions and 2 cases were 3 lesions .15/16 cases with malignant lesions were detected significant 18F-FDG uptake ,only 1 case with bronchioloalveolar cell carcinoma was mild uptake,In 18F-FDG scans, in patients with, the average SUVmax of malignant lesions was 9.19±4.75, and the benign lesions was 3.05±1.18; The uptake of lung lesions on 18F-FLT is generally lower than 18F-FDG, in 18F-FLT scans, the average SUVmax of malignant lesions was 3.39±1.27, the benign lesions was 1.45±0.64; The SUVmax were significantly different between the benign and malignant lesions on 18F-FDG as well as 18F-FLT (P<0.05). Of the 4 cases of benign lesions, only 1 case was found high uptake either in 18F-FDG or 18F-FLT imaging. In≤3 cm lesions group ,the average of 18F-FDG SUVmax was 6.40±3.04,the value was 2.50±1.13 for 18F-FLT;In >3 cm lesions group , the average of 18F-FDG SUVmax was 11.40±5.86,and the value was 4.11±1.22 for 18F-FLT; Both 18F-FDG and 18F-FLT imagings were higher uptake in > 3 cm lesions group (0.01 |
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