Inhibition Of Transforming Growth Factor-β1-induced Transdifferentiation By Connective Tissue Growth Factor Antisense Oligonucleotide In Human Lens Epithelium Cells

ObjectiveTo investigate the influence of CTGF-ASON on transdifferentiation induced by transforming growth factor-β1 (TGF-β1) in normal human lens epithelium cells(HLECs).Methods(1)CTGF-ASON was transduced into normal human lens epithelium cells by co-transfection with Lipofectamine 2000 or direct ways. The transfection ratio of FITC-labeled ASON was measured by fluoroscope. (2)the proliferation of HLECs was measured by CCK-8 assay. (3)The expression of CTGF andα-SMA was detected by RT-PCR. (4)The expression of CTGF andα-SMA was detected by cell immuno chemical staining.Result(1)Direct ASON transfection was slow, and no obvious cytotoxity was observed within 72h. ASON treated for 48h, The transfection ratio of FITC-labeled ASON run up to 63%. (2)The proliferation of HLECs was not inhibited by direct ASON transfection until 72h. (3)ASON treated for 48h, Culture in TGF-β1 caused profound increase of CTGF andα-SMA that can be inhibited by direct ASON transfection, but not missense oligonucleotides transfection. ConclusionCTGF-ASON can abrogate the influence of TGF-β1 on increase of CTGF andα-SMA in HLECs. CTGF may be a potential therapeutic target to Posterior Capsular Opacification.