| Fluorescence spectra of active components in Caulis Erycibes (CE, Dinggongteng in Chinese) and Flos Lonicerae Japonicae (FLJ, Jinyinhua in Chinese) were studied in this thesis, and influences of experimental conditions on fluorescence spectra were also investigated to establish the best conditions. Solution fluorescent method and thin-layer fluorescence scanning method were established for the determination of scopoletin. The content of Chlorogenic Acid (CA) in FLJ was determined by solution fluorescent method.Five parts were included in this thesis:1. Fluorescence spectra of scopoletin and scopolin in CE were studied. It was found that solution acidity had great effect on the fluorescence spectra of scopoletin, and had no effect on scopolin. The fluorescence quantum yields of scopoletin and scopolin were measured to be 0.71 and 0.21 respectively, using quinine sulfate as a reference. The ionization constant of scopoletin was determined to be pKa = 7.32, by using a fluorimetric method.2. Fluorescence spectra of the extract of CE were studied. Solution fluorescent method was established on the basis of difference between scopoletin and scopolin in the change of solution acidity. And thin-layer fluorescence scanning method was also established.3. Fluorimetric method as well as thin-layer fluorescence scanning method was established to determine the total content of scopoletin in CE by transforming scopoletin into scopolin.4. The fluorescence of CA was weak. So, method for obtaining sensitized fluorescence of CA was explored. It was found that the fluorescence of CA can be sensitized by coexisting with borax and CTAB. Fluorescence spectra of CA-borax -CTAB ternary system was then studied, and the fluorescence quantum yield of the ternary system was measured to be 0.010, using quinine sulfate as a reference.5. The fluorescence components in FLJ were studied. Fluorimetric method for the determination of total content of CA was established using borax-CTAB as the sensitizing agent. |
PDF Full Text Request