The Expression Of Intestinal Dc And Constimulatory Molecules CD80,CD86 In The Rat Model With Intestinal Dysfunction Induced By Acute Stress

Irritable bowel syndrome is the most common functional intestinal disorders,of which the pathogenesis is not yet clear.Many studies show that the existence of intestinal inflammation in intestinal of the patients with irritable bowel syndrome.In recent years,we pay attention to the disorders about Nerve-Immune-endocrine network. Therefore, to establish the rat model with acute stress as an incentive and the change of Faeces Characters as Features,to discuss the etiology and pathogenesis of IBS,whitch is of important necessity and feasibility of the experiment.The animal experiments have shown that stress may be an important factor in inflammation.we observe the expression of rat intestinal DC,costimulatory molecules CD80,CD86 and VIP,IL-1β,and also discuss the functional change of rat intestinal immune tolerance,in order to provided a new direction to study the pathogenesis and treatment of IBS through the rat model with intestinal dysfunction induced by cold-restraint stress.Objective: we observe the expression of rat intestinal DC,costimulatory molecules CD80,CD86 and VIP,IL-1β, and the levels of VIP,IL-1βin peripheral blood through the rat model with intestinal dysfunction induced by cold-restraint stress,in order to discuss the functional change of rat intestinal immune tolerance.Methods The 40 wistar rats were randomly divided into control group and experimental group.We establish the rat model with intestinal dysfunction induced by cold-restraint stress. During the trial period,,the stool of two groups were scored according to the Bristol pattern,and colorectal distention was implemented to test the visceral sensitivity in 6th day. In 7th day,pathological changes of the the terminal ileum and distant colon mucosa in two groups were measured,and immunohistochemistry﹙S-P method﹚were used to detect the expression of intestinal surface DC,constimulatory molecules CD80 ,CD86, and enzyme-linked immunosorbent assay(ELISA) were used to detect the expression of VIP,IL-1β.VIP,IL-1βin peripheral blood.Results Compared with control group,the stool character of experimental rats had changed from the sixth day, and the sensory threshold ofintestinal were evident decreased(P<0.05); There were no significant difference between the two groups through the macroscopic and histological score;The expression of DC,CD80,CD86 in terminal ileum was similar to that of controls(P>0.05);whereas the expression of VIP,IL-1βin terminal ileum was markedly increased compared with control group (P<0.05),and positive liner correlation between VIP and IL-1βwas observed in terminal ileum(r=0.78,Pα<0.01).In distant colon, the expression of constimulatory molecules CD80,CD86 and VIP was markedly increased compared with control group (P<0.05),but the expression of CD11c and IL-1βin distant colon was similar to that of control(sP>0.05). and positive liner correlation between VIP and CD80 was observed(r=0.70, Pα<0.01),the positive liner correlation between VIP and CD86 was observed in distant colon(r=0.34, Pα<0.05). The expression of blood VIP,IL-1βwere all increased significantly compared with control group (P<0.05).Conclusion There have been low grade inflammation in the terminal ileum and distant colon of the rat model with intestinal dysfunction induced by cold-restraint stress. The existence of mild intestinal inflammatory was verifyed by the expression of constimulatory molecules CD80,CD86and DCs from the perspective of immune response. We speculate that CD80,CD86 in distant colon may play an important role in intestinal immune regulation after intestinal dysfunction,and may be the biological indicators of intestinal dysfunction.