Effect Of Exercise And Chronic Stress On The Behaviors, Immunological Function And Neuroendocrine Function Of Rats

Objective:By observing the effect of exercise and choronic stress on change of mood, behaviors, function of learning, function of memory and recognition, and the level of IL-1β,IL-2, ACTH, CORT, the express of 5-HT of mice, this paper aims to explore the effect of exercise and chronic stress on psychological behavior, immunological function, neuroendocrine function of mice. It is expected that this research would be of great significance in guarding against sub-health, adopting effective psychological intervention measures and improving people's living standard.Methods:40 SD mice were randomly divided into control group,chronic stress group, exercise group,stress and exercise group.Each group had 10 mice,five mice are male,five are female.This model used four stressor:bounding mice, tilting mice cage,merging mice, prohibiting food and water. Then adopted swimming to intervene chronic psychological stress mice. Implemented Open-field every week; Morris water maze and Suspend tail test at the last week to observe the change of mood and behaviors, and function of learning, and function of memory and recognition in mice. ELISA was employed to measure levels of IL-1β, IL-2. RIA was employed to measure levels of ACTH, CORT. Expression of 5-HT in neural cells in rats' brain tissue was measured with ABC of immunohistoc-hemical method.Results:In open field test, stress group mice had relatively longer latency time(P＜0.01) and lower scores (P＜0.01) than control group; exercise and stress group mice had relatively shorter latency time (P＜0.01)and higher scores (P＜0.01)than stress group. In suspend tail test, compared with control group, stress group mice had relatively longer staying times (P＜0.01) and fewer struggle times(P＜0.01).There was no difference of statistical significance between stress group and exercise and stress group (P＞0.05).In hidden-platform training of Morris water maze, the mean escape latency of the mice in the stress group were longer than that of the control mice(P＜0.01) and stress and exercise group(P<0.05). In spatial probe tests, the average percentages of the swimming time in the platform quadrant in the stress group mice were less than those in the control group(P＜0.01) and stress and exercise group(P＜0.05). The difference of spleen index and thymus index of rats in four group all had no statistical significance (P＞0.05).Compared with the control group, the IL-1β, IL-2 contents in stress group decreased significantly(P＜0.01), the ACTH, CORT contents in stress group significantly increased (P＜0.01). Compared with the stress group, the IL-1β, IL-2 contents in stress and exercise group were significantly increased (P＜0.01), the ACTH, CORT contents in stress and exercise group were significantly decreased(P＜0.01).Conclusion:It is showed that chronic stress can result in anxiety and depression, and descent of learning, memory, and cognition function. Furthermore, chronic stress is able to suppress immunological function and neuroendocrine function of rats. Nevertheless, exercise can ameliorate anxiety and depression, and suppression of learning and memory, and cognitive changes in chronic stress mice. Meanwhile, exercise has protection function to chronic stress mice and can improve immunological function and neuroendocrine function of rats.