The Effect Of Alarelin And 5-Fu On The Inhibition Of The Transplanted Endometrial Tumor Of The Nude Mice And The Expression Of Bcl-2/Bax

Background and objectives:Endometrial cancer is the malignant epithelium cancer from the endometrium, it accounts for 20%～30% malignant tumor in female reproductive system. The incidence is going up in recent years. The treatment consist of the operation (the major treatment), and the other adjuvant treatment, such as hormone treatment, radiotherapy, and chemotherapy. The clinical therapeutic effect of the chemotherapy was obviously decreased because of drug resistant. The part recurrence could be decreased after the operation by radiotherapy, but the survive rate of the patient suffering from endometrial cancer can't be improved and the distant recurrence could't be prevented after the operation either. In the hormone treatment, the side effect is reduced, the toxicity is lower and it is easily controlled, but the dosage and method isn't united. So there are important significance to seek the new treatment.The hormone tratment got more and more attention and research since the morbidity of endometrial cancer was related with the long-term stimulation of estrogen on the endometrium, and the research got a big advance. The research about the breast carcinoma, ovarian cancer and prostatic carcinoma showes gonadotropin releasing hormone analogue (GnRH-a) inhibit the tumor cells. Alarelin is nine peptides analogs of GnRH-a, The research revealed that alarelin can modulate the part related oncogene of endometrial cancer. But the medicine mechanism of GnRH-a on the transplanted endometrial tumor of the nude mice and the rational use of drug was seldom investigated.The model of the transplanted endometrial tumor of the nude mice was established in the study,the expression of Bcl-2 and Bax and the change of endometrial cancer after exposed to alarelin and 5-Fu were observed to investigate the apoptosis of endometrial cancer cell,Methods:The the model of the transplanted endometrial tumor of the nude mice was established, the nude mice carrying tumor were divided 4 groups, including the control group, alarelin group(35mg/kg),5-Fu group(5mg/kg) and the therapeutic alliance group(alarelin and 5-Fu). The nude mice were sacrificed, and the tumor weight were weighed, the cell morphology of the transplanted endometrial tumor were obseved by HE, and the expression of Bcl-2,Bax were examined by immunohistochemistrySABC.Results:1. The growth of transplanted tumor was inhibited at different degree in alarelin group and 5-Fu group.The combination effect was more obvious. The rates of the tumor inhibition were 21.02%,31.44% and 55.95% in alarelin group,5-Fu group and the combination group.2.The expression of Bcl-2 decreased, and more significant in the therapeutic alliance group.3.The apoptosis cells were more in the therapeutic alliance group than other groups.Conclusions:1. Alarelin could inhibit the growth of the transplanted endometrial tumor in the nude mice.2.Alarelin could inhibit endometrial HEC-1B cells by decreasing the expression of Bcl-2 and promoting the apoptosis of endometrial cancer cell.3.Alarelin and 5-Fu played the synergistic effect when both were combined on the treatment of endometrial cancer.4. Alarelin have no obvious side effect, it could increase the weight of the nude mice carrying the tumor and improve the lose of body mass.