The Protection Of NK1 Receptor Antagonist L-703,606 Following Traumatic Brain Injury In Rat

Objectives:To investigate the protection of NK1 receptor antagonist L-703,606 after traumatic brain injury in ratMethods:seventy adult male Wistar rats were randomized into control group, traumatic brain ingury group and L-703,606 group.The model of free falling body was proceed in TBI,and L-703,606 groups.The group of L-703,606 was immediately administrated NK1-receptor antagonist L-703,606 by vena caudalis after giving traumatic brain injury in right parietal lobe, TBI group was not treated, control group was Opened the skull translation in the same place and Exposed dura, Bone window was closed by Bone wax. Scalp was sutured After bleeding was stoped, Brain was not impacted. All groups was taken the brain at 0.5,2,6,12,24,48,72 h. Immunohistochemistry method were used to observe and detect the changes of SP on specific time spots 0.5,2,6,12,24,48,72h in control group,TBI,and L-703,606 group.Results:Immunohistochemistry showed that positive unit of SP in TBI group was up-regulated 0.5 hour after injury, progressively enhanced from 2h to 6 h, peaked at 12 h,and descend in the rest time, remained a higher level at 72 h.Unlike the TBI groups,the expression of positive unit of SP in L-703,606 groups was peaked at 6 h, continuing descended in the subquent time,recoveried at 72h(P<0.05)。The positive unit of SP in L-703,606 groups at the same time was less than the TBI groups. They also have significant difference (P<0.05)。hey have different positive unit of SP between control groups, TBI groups and L-703,606 groups. Statistics showed they have significant difference (F=74.181, P<0.01)。The control groups did not have significant difference among 0.5,2,6,12,24,48,72 h (P>0.05)。Generally,the expression of positive unit of SP in L-703,606 groups and TBI groups had significant difference at 0.5,2,6,12,24,48,72h(P<0.01)。The positive unit of SP in TBI groups at 6,24,0.5,48 h did not have significant difference(P>0.05)。Time and packet had Interaction effect. (F=3589.696, P<0.01)。Conclusion:NK1 receptor antagonist L-703,606 can reduce the expression of SP following traumatic brain injury in rat,decrease brain edema and hasten recovery.