Expression And Related Research Of HPV16/18 CD44v6, Galectin-3 In Esophageal Cancer

Objective To investigate the expression of the human papilloma virus 16/18 (HPV16/18), adhesion molecules CD44v6 and agglutininβ-galactoside-binding protein-3 (Galectin-3) in esophageal cancer and explore their correlativity.Methods The expression of CD44v6, Galectin-3 and HPV16/18 in 72 patients with esophageal cancer,18 cases of normal esophageal mucosa,12 cases of low-grade intraepithelial neoplasia,18 cases of high-level intraepithelial neoplasia were detected by immunohistochemical S-P technique and in situ hybridization.At the same time, relationship between CD44v6, Galectin-3, HPV16/18 and esophageal cancer was explored.Results 1.The positive rate of CD44v6 was 50% (36/72) in esophageal cancer.The positive rate of CD44v6 in low-grade intraepithelial neoplasia was significantly lower than that in high-grade intraepithelial neoplasia and cancer.There was statistical significance,and the difference was significant(P<0.01).The positive rate of CD44v6 in normal esophageal epithelium was significantly lower than that in high-grade intraepithelial neoplasia and esophageal cancer.There was statistical significance,and the difference was significant(P<0.01);and the positive rate of CD44v6 in high-grade intraepithelial neoplasia was higher than that in esophageal cancer, and there was statistical significance (P<0.05). The positive rate of CD44v6 in phaseⅡA ,ⅡB was 57.89%(22/38),23.08%(3/13) respectively,there being statistical significance (P<0.05).However, clinical staging of esophageal cancer,the positive rate of CD44v6 inⅠandⅡA period of esophageal cancer,ⅡB,Ⅲor,Ⅳwas not statistically significant (P>0.05).The expression of CD44v6 had no correlation with esophageal cancer clinical stage, histological grade, the morphology of gross specimen, lymph node metastasis, sex, age and depth of invasion but some correlation with the malignant transformation of esophageal squamous epithelium.2.The positive rate of Galectin-3 was 58.33%(42/72) in esophageal cancer.The positive rate of Galectin-3 in low-grade intraepithelial neoplasia was significantly lower than that in high-grade intraepithelial neoplasia, there was statistical significance(P<0.05).The positive rate of Galectin-3 in normal esophageal epithelium was significantly lower than that in the high-grade intraepithelial neoplasia, there was statistical significance (P<0.05).And the positive rate of Galectin-3 in the high-grade intraepithelial neoplasia was higher than that in esophageal cancer,there was statistical significance(P<0.05).The expression of Galectin-3 had no correlation with esophageal cancer clinical stage, histological grade, the morphology of gross specimen, lymph node metastasis, sex, age and depth of invasion but some correlation with the malignant transformation of esophageal squamous epithelium.3.The positive rate of HPV16/18 was 55.56% (40/72) in esophageal cancer.The positive rate of HPV16/18 in normal esophageal epithelium was significantly lower than that in esophageal cancer, there was statistically significant (P<0.05).The positive rate of HPV16/18 in the high-grade intraepithelial neoplasia was lower than that in esophageal cancer,there being statistical significance(P<0.05).The positive rate of HPV16/18 in the low-grade intraepithelial neoplasia was significantly lower than that in esophageal cancer.There was statistical significance, and the difference was significant(P<0.01).The expression of HPV16/18 had no correlation with esophageal cancer clinical stage, histological grade, the morphology of gross specimen, lymph node metastasis, sex, age and depth of invasion but some correlation with the malignant transformation of esophageal squamous epithelium.4.The expression of HPV16/18 and Galectin-3, CD44v6 were positively correlated with the clinical stage, indicating the three factors in the development of esophageal cancer have a certain synergy.Conclusion 1.Both CD44v6 and Galectin-3 has correlation in the development of esophageal carcinoma. 2. HPV16/18 has a certain relevance in the development of esophageal carcinoma and can be used as a cause of esophageal cancer preventive therapy.