Study On The Correlation Between Endothelial Microparticles And Alzheimer's Disease

【Background】Alzheimer's disease (AD) is a kind of dementia that owing to chronic progressive nervous system degeneration, which is the most commonly etiopathogenisis of dementia and the most frequency of gerontic dementia. in recent years, as the pathogenesis of Alzheimer's disease has continuously studied, discovered that the risk factor of blood vessel is playing a more and more important role in the pathogenesis of AD and it's advancing. CD31+/CD42- endothelial microparticle(EMPs) is a available index to reflect the function disorder of endothelial cell in recent researches, so we dectcted the number of CD31+/CD42- EMPs in the AD patients and the mild cognition impairmen(tMCI)patients, to study the relationship between endothelial dysfunction and Alzheimer's disease.【Objective】Dectction the number of CD31+/CD42- EMPs in Alzheimer's disease, to study the relationship between endothelial dysfunction and Alzheimer's disease, and measuring scales of dementia were assessment to AD patients and the mild cognition impairment(MCI)patients. To analysis the relevance between the number of CD31+/CD42- EMPs and the score of measuring scales.【Methods】Choose 30 cases of AD patients (consistent with the diagnostic criteria of National Institute of Neurological and Communicative Disorders and Stroke and the Alzheimer Disease and Related Disorders Association),15 mild cognition impairmen(tMCI)patients which without vascular risk factors and 30 healthy people, detect the level of CD31+/CD42- EMPs in peripheral blood by flow cytometry, and measuring scales of dementi(aADL,CDR,MMSE,HIS and ADAS-cog et.al) were assessment to AD patients and the mild cognition impairment(MCI)patients, To study whether the level of CD31+/CD42- EMPs is increase obviously between patients and healthy people. Collect the clinical data include age,gender,age of onset,Sick time and the time take medicine, and study the relevance between the level of CD31+/CD42- EMPs and the clinical data separately. Finally, all parameters which related to the level of CD31+/CD42- EMPs(all parameters are independent variables and the level of CD31+/CD42- EMPs is dependent variable) progressing multiple stepwise regression, analysis whether they also can play a role in the multi-factor analysis, use calculate normalization partial regression coefficient to decide the most important factor.【Results】1, In the AD patients , the level of CD31+/CD42- EMPs were significantly higher than the healthy control group (P <0.01); In the MCI patients without vascular risk factors, the level of CD31+/CD42- EMPs has no significantly increase than the healthy control group (P >0.01), but has Increasing tendency. There has positive correlation between the level of CD31+/CD42- EMPs and the degree of dementia in AD patients.2 ,The clinical data include age,age of onset,Sick time,the time take medicine,diastolic pressure,fibrinogen and blood platelets count have no correlation with CD31+/CD42- EMPs, the score of ADL,ADAS-cog and CDR , fasting blood sugar,cholesterin and triglyceride have positive correlation with CD31+/CD42- EMPs, and the score of ADAS-cog is the most important factor that correlate with the level of CD31+/CD42- EMPs.【Conclusion】1 ,In the AD patients , the level of CD31+/CD42- EMPs were significantly higher than the MCI patients without vascular risk factors and the healthy control group (P <0.01),In the MCI patients without vascular risk factors, the level of CD31+/CD42- EMPs has no significantly increase than the healthy control group (P >0.01), but has Increasing tendency. AD patients has functional disorder of endotheliocyte. 2, There has positive correlation between the level of CD31+/CD42- EMPs and dementia degree in AD patients. The level of CD31+/CD42- EMPs can used to assessment the degree of dementia severity in AD patients.