The Expression And Ovary Hormonal Control Of Growth Factors On Endometrium

Objective:Implantation, initiated by direct contact between a hatched embryo and the surface of uterus, which is a complex developmental process. Successful implantation results from a co-ordinated series of events that allow establishment of a timely dialogue between a receptive endomertrium and an invasive blastocyst.The secretion peak of some cytokines coincidents with the implantation window. The cytokines are involved with the regulation of embryo development and implantation by autocrine and paracrine ways. For example, high concentration of LIF can accelerate the growth of trophoblast cells and stem cells, and also conduce to transferred embryo implantation and survival. Implantation is significantly inhibited by blocking LIF with LIF-Ab. The results indicate that cytokines may act as mediators during fetal-mother recognition.It is known that growth factors are highly expressed on the cell surface of endometrium and blastocyst during the mice implantation stage. Growth factors involved in reproductive regulation, ovulation, embryo development, blastocyst implantation, and embryonic stem cell differentiation, play an important role, and this role has attracted people's attention so far.Steroid hormone plays a critical role during implantation. To investigate the correlation between growth factors and steroid hormone, analysis of whether steroid hormonal regulate growth factors'expression of embryo and uterus during implantation is implemented.Methods:In this essay, the expression of growth factors on human and mice endometrium in various hormonal levels, and the regulating mechanicism of growth factors are investigated by RT-PCR and Immuno-histochemical staining. The mice were selected and treated as follows, (1) Early pregnancy:females were mated with males (1:1); and the day of vaginal plug appearance was considered as day 1 of pregnancy; (2) Hormone replacement after ovariectomy:mice were divided into 4 groups (control group, estrogen group, progestin group, and estrogen and progestin group). RT-PCR and immunohistochemical analysis were used to examine the effect of steroid hormone on the expression of the growth factors.Results:Immunohistochemical revealed that EGF,EGFR, TGF, VEGF and so on, expressed in human endometrium the obvious staining of epithet-lium in secretory phase was darker than that in proliferative phase,which may be controlled by ovarian hormones. RT-PCR detected the expression of the growth factors during early pregnancy(D1), the level of growth factors was lower, from D3 to D5 was higher, and highest on the surface of uterus during implantation. In the mice that were treated with hormone after ovariectomy, the expression of the growth factors was regulated by estrogen and progestin hormone, the roles were similar, that was to say, down-regulated by estrogen and up-regulated by progesterone, the two hormones antagonized each other and influenced the expression of the growth factors.Conclusion:Growth factors express in the endometrium, ovary hormone controls the expression of growth factors on the transcription level.