Expression Of 5-hydroxytryptamine In Hippocampus CA1, CA2 And CA3 Of Rats

Objective:Hippocampus of rats blongs to of limbic system brain, including CA1, CA2 and CA3. It is no significant boundary in the structures, and function can be considered as a whole.5-hydroxytryptamine (5-HT), a kind of wide neurotransmitter in the central nervous system have mainly involved in emotion, sleep, pain, learning and memory and other complex regulation of physiological functions。In order to understand the 5-HT in the hippocampus of the distribution, the experiments was held, observed in hippocampus CA1, CA2 and CA3 three regional the positive neurons and 5-HT positive nerve fiber terminals in the expression, for further study of 5-HT in the hippocampus learning and memory mechanism, providing morphological evidence.Method:1,The experimental animals and main reagents:8 adult male SD rats (from the Experimental Animal Center of Dalian Medical University).Polink-2 plus polymer HRP detection system for goat primary antibody.and 3,3,-Diaminobenzidine tetrahydrochloride substrate kit, Immunohistochemistry kit (from ZSGB-BIO) 2,perfusion and fixation:the rats were taken firstly based anesthesi in the cylinder by Ether, and then was carried out intraperitoneal anesthesia by chloral hydrate(10%), Opened the chest with scissors, with the puncture needle from the left ventricle to ascending aorta, cut the right atrial appendage, first washed with 150ml NaCL(0.9%) quick and perfusated paraformaldehyde(4%) in PBS 300ml, was cut cranial brain and placed in the PBS overnight at 4℃. It was cut into slice serial coronal sections in the vibrating slicer, it be selected the slice with the hippocampus, placed in 0.01mol/L of PBS(PH7.4) solution. 3,immunohistochemistry:The experiments was used to be two-step immunohistochemical staining. 4,statistical analysis:It was observed In Nikon (ECLIPSE80i) by fluorescence microscope image analyzer and the image analysis software with their own analysis of the data. Localization of nuclei of light depend on The Rat Brain in stereotaxic coordinates (third edition).It was observed that the hippocampal area and the 5-HT-positive nerve cells at the low microscope, It was counted 5-HT-positive neurons in the hippocampus CA1, CA2 and CA3 areas in the 10×20 light microscope.It was Observed that 5-HT positive nerve fiber terminals at high he low microscope.The data analysises with the soft SPSS16.5.Result:At low light microscope:1.The 5-HT immunoreactive product was brown in the hippocampus,a large number of 5-HT positive neurons can be discoveried, positive products were located around the nucleus, the nuclear transparency has not significant stained.2.Immunoreactive cells was band in the hippocampal pyramidal layer of the distribution 3.Counting in 20×10 light microscope immunoreactive cell bodies of CA2 area is the most, followed in the CA1 area, CA3 area are least. there are significant differences by statistical analysis among the CA1, CA2 and CA3 area the number of positive neurons, CA1>CA3, CA2>CA3 (P<0.01).4.The cells of CA3 area is larger than other regions, but weaker staining than the CA1 area. At the high microscope: 1.It shows that more immune 5-HT-positive nerve fibers and terminal 5-HT nerve fibers were beaded terminals (Figure 2b,3b, 4b).2.The positive neurons were round or oval.Otherwise,at the light microscope,the control sections have no expression of anti-5-HT immunoreactive cell body and anti-5-HT immunoreactive nerve fiber terminals in CA1, CA2 and CA3 areas.Its mean values are 5.60±1.075, 5.70±1.252,3.50±1.269.Conclusion:1.There exist 5-HT nerve fiber terminals and cell bodies in the CA1,CA2 and CA3 of hippocampus.2.The expression of 5-HT immunoreactive products in CA1, CA2 and CA3 areas is uneven, the number of positive neurons is CA1>CA3,CA2>CA3.We conclude that 5-HT neurons may play a role as interneurons in hippocampus...