Influence Of FTY720 On Neuronal Microenvironment And Apoptosis Of Acute Spinal Cord Injury In Rats

Acute traumatic spinal cord injury is a serious disease, has been lack of effective treatments. In recent years, with the mechanism of spinal cord injury researching, the autoimmune response gets more attention. By immunosuppressive therapy to improve spinal cord injury microenvironment, and promote functional recovery, as treatment of spinal cord injury in a new way. Following a strong long after, it was found a series of immunosuppressive drugs are treatment of spinal cord injury, But the immune suppression caused by systemic side effects of drugs seriously affect their promotion and application in clinical practice. FTY720, the chemical name 2-Amino-[2-(4-octyl-phenyl) ethyl]-1,3-propanediol hydrochloride, is the medium from the Cordyceps sinensis extract the antibiotic composition ISP-1, the chemical modified synthetic, is different from the other new immunosuppressants. FTY720 on human toxicity, peripheral blood neutrophils was no significant decrease, not the liver, causing significant damage to kidney function, immune function in maintaining the same time, you can effectively play its role in immune regulation. New research shows that, FTY720 on brain neurons has a therapeutic effect, the specific mechanisms remain unclear. However, FTY720 whether neurons in acute spinal cord injury has a protective effect, not yet known. The issue in the establishment of experimental animal models of acute spinal cord injury based on the use of FTY720 intervention on injured animals, from the micro-environment and neuronal apoptosis in the perspective of new immunosuppressive agents on spinal cord injury in rats.Objective:FTY720 used in acute spinal cord injury in animal models of acute spinal cord injury in their micro-environment of neurons and the neuronal apoptosis in vitro.Methods:84 female SD rats were randomly divided into sham operation group (A group), model group (B group) and FTY720 treatment group (C group), which groups 14 A, B group and C group 35. Sterile conditions, the modified Allen'S T9 combat established animal model of spinal cord injury, sham operation group laminectomy. FTY720 treatment group by 3mg/kg diluted with normal saline, administered once to take 0.3mL, sham operation group and model group were administered with normal saline. Rats in each group were healed at 6h,12h,24h,48h,72h, 1w, 3w drawn, drawn at different time points in each group 5, were performed pathological microenvironment, immunohistochemical staining the expression of caspase-3, Tunel assay apoptosis and immunofluorescence positive cells. Image data using Image-pro plus 6.0 image analysis processing, data processing using SPSS13.0.Results:The injury 6h,12h,24h,48h,72h, 1w,3w FTY720 treatment group (C group) spinal cord hemorrhage in necrotic area and inflammatory cell infiltration compared with model group (B group) significantly reduced; caspase-neuronal cells 3 in volume, neuronal apoptosis, and the number of neurons staining positive cells compared with model group (B group) was significantly reduced, the difference was significant (P<0.05); sham operation group (A group) of the above are better than the model group (B group) and FTY720 treatment group (C group).Conclusion:FTY720 on acute spinal cord injury in neuronal cells has a protective effect; FTY720 is acute spinal cord injury by reducing inflammatory cell infiltration, inhibition of neuronal apoptosis, and then play a relieve spinal cord injury; FTY720 inhibited after acute spinal cord injury Caspase-3 expression and neuronal apoptosis in the specific mechanism needs further study.