Research On Preparation And Anti-hepatoma Of Docetaxel Carried Carbon Containing Iron Nanoparticles

Hepatoma is some kind of a frequent tumor with highly malignant biological behaviour and a fairly bad prognosis.The inhabiting effect of conventional chemical therapy on hepatic cell carcinoma is definite. During the chemical drug on primary hepatoma, routine cytotoxic drug used in chemical therapy has a fairly good deal of toxicity and low specificity,and these kind of drug can bring severe impairment to hepatic and others organism.Therefore the research on chemical cytotoxic drug used in therapy of primary hepatic cell carcinoma mainly concentrates on the route and method of administration. Administration of chemical therapy after hepatic artery catheterization has a bad effect. Nowadays, there are constantly more and more new clinical anti-cancer drugs, docetaxel (DTX) is a kind of anti-cancer drug synthesized on the base of taxinol,and it was once considered the most effective mono-drug in chemical therapy,however as cytotoxic drug its damage of other cells except for carcinoma cells can't be avoided.these years,the advancement of molecular targeting technology bring new chance to the clinical therapy of hepatoma,the development of nanometer technique and the continuous application of nanometer meterial carrying chemical therapy drug using in research on tumor have got satisfactory effect.We do research on carbon coated iron nanoparticles (CCINs) carrying DTX,and observe its killing effect on hepatoma cells.Thus providing evidence in approaching new pathway of hepatic-cell-carcinoma-therapy and elevating applicating effect of docetaxel.Chapter oneResearch on preparation of carbon containing iron nanoparticles carrying docetaxelResearch objectiveInvestigate effect of CCINs carrying DTX, estimating its physical and chemical characters,discussing and investigate superordinary preparing technology of CCINs- DTX.Research methodsTo Carry DTX in CCINs with supersound emulsification- dissolvant technology,to evaluate its carrying weight to DXT, wafer rate and efficacy of drug release with colorimetric method,and investigate its magnetism with vibrating sample magnetometer(VSM),and to inspect its stability with laser particle size analysator.Research consequenceAdopt supersound emulsification-dissolvant technology to composit CCINs-DTX, then detecting showing that its carrying-drug weight is 274mg/g, Encapsulation rate is 82.74%,it hint that DTX can get effective concentration fastly and keep stable drug concentration..The detection of the stability of CCINs-DTX shows that it has satisfactory stability,and particle diameter was invariant in three months,and has good magnetic efficacy that was found by detecting.Research conclusionThe CCINs-DTX prepared through supersound emulsification-dissolvant compositing technology has good carrying-drug weight and Encapsulation rate,releasing efficiency and stability,and has good magnetism.chapter twoThe impact of CCINs-DTX acting on extracorporeal-cultivated hepatoma cells on its growth and apoptosis. Research objectiveTo observe the impact of CCINs-DTX acting on extracorporeal-cultivated hepatoma cells on its growth and apoptosis,discussing its mechanism of anti-tumor effect.Research methodRandomly dividing the extracorporeal-cultivated hepatoma cells into four groups,the drug group (DTX), particle-drug group (CCINs-DTX),blank group (NS) and blank particle group (CCINs). interfering the drug group with DTX, interfering the particle-drug group with CCINs-DTX,interfering the blank group with isotonic Na chloride,interfering the blank particle group with CCINs,adopting chromatometry to detect the growth-inhabiting rate of cells of every group,adopting flow cytometry to detect adoptosis exponent of cells of every group,Comparing the growth-inhabiting rate and adoptosis exponent.Research consequenceIn comparison with the drug group, growth-inhabiting rate of hepatoma cells was identical in the particle-drug group when drug concentration was lesser than 80mg/L,and growth-inhabiting rate was higher in the particle-drug group when drug concentration was more than 80mg/L at 24h,48h and 72h.In comparison with the blank group, hepatoma cells did not shown growth inhibiting in the particle group.In comparison with the blank group and the particle group, adoptosis rate of hepatoma cells were increasing in the drug group and the particle-drug group,in comparison with the drug group, adoptosis rate were increasing in the particle-drug group, in comparison with the blank group, adoptosis rate of hepatoma cells was identical in the particle group.Research conclusionCCINs-DTX had a obvious inhabiting effection on extracorporeal-cultured hepatoma cells,and it could has anti-tumor effection by leading to the apoptosis of cells.